Antimalarial agents

ABSTRACT

The present invention provides methods of treating malaria comprising administration of compounds of Formula (I) or a pharmaceutically acceptable salt thereof, to a subject in need thereof, wherein the variables are as defined herein. The invention also provides uses of the compounds of Formula (I), as defined herein, for the possible inhibition of plasmepsin X, plasmepsin IX or plasmepsin X and IX activity, for treating a Plasmodium infection, and for treating malaria. Also provided are methods of treatment further comprising administration of one or more additional anti-malarial compounds.

FIELD OF THE INVENTION

The present invention relates to compounds of Formula (I), or pharmaceutically acceptable salts thereof, useful for the treatment of Plasmodium infections. More specifically, the present invention relates to compounds of Formula (I), or pharmaceutically acceptable salts thereof, useful for the treatment of Plasmodium infections, more particularly for the treatment of malaria.

BACKGROUND OF THE INVENTION

Malaria is a major disease in humans with several hundred million infections and over 450,000 deaths each year. The most lethal form of malaria is caused by Plasmodium falciparum. This protozoan parasite is responsible for almost all malarial deaths with most occurring in Africa. P. falciparum has a complex life cycle starting in the Anopheles mosquito vector when sporozoite forms are injected into the human host during a blood feed. These sporozoites migrate to the liver and invade hepatocytes in which they develop to form thousands of liver merozoites that egress into the blood where they invade erythrocytes to commence the asexual cycle of the parasite responsible for the symptoms of malaria. The parasite develops within the protected niche of the red cell to form 16-32 merozoites that, once mature, egress from the host cell to invade new red blood cells. Some of these parasites differentiate to form gametocytes, the sexual form of the parasite. These can be taken up by the mosquito where male and female gametes form, fuse and differentiate into oocysts on the mosquito midgut extracellular matrix. Sporozoites form within the oocyst and upon egress migrate to the salivary gland for delivery to the next host during blood feeding for perpetuation and survival of the parasite.

Other forms of malaria include a relapsing form of malaria caused by P. vivax which is responsible for significant morbidity, can cause virulent forms of this disease with some deaths and is mainly a problem outside Africa. P. knowlesi is found in South East Asia and is a zoonotic parasite that normally infects long-tailed macaques but has been shown to infect humans in Malaysian Borneo.

Artemisinin combined with partner drugs have become a mainstay in the treatment and control of malaria. Currently, the WHO recommend artemisinin-based combinations (ACTs) for treatment of uncomplicated malaria caused by P. falciparum. These are very effective; however, ACT resistance is now leading to treatment failures in the Greater Mekong Subregion (GMS) and the prospect of spread outside this region is extremely concerning. However, due to the increasing threat of artemisinin-based combination therapy (ACT) drug resistance, the development of new antimalarials with novel targets that inhibit multiple steps in the parasite life cycle is an urgent priority for the malaria control field. Such novel antimalarials, as monotherapies or ACT partner drugs, could make strides towards malaria elimination as there is a reduced likelihood of parasites with preexisting resistance mutations being present in the parasite population.

Currently, aspartic acid proteases are prime targets for drug development: the HIV aspartic acid protease has been successfully targeted with a drug in clinical use; inhibitors that target human renin, BACE1 and gamma-secretase have been or are in clinical development. In the antimalarial drug space, P. falciparum aspartic acid proteases plasmepsin X and IX (PMX and PMIX) have been identified as potential targets since inhibitors block parasite egress and invasion of the host cell and prevent maturation of some rhoptry and micronemal proteins required for this process (Pino P, Caldelari R, Mukherjee B, Vahokoski J, Klages N, Maco B, et al. A multistage antimalarial targets the plasmepsins IX and X essential for invasion and egress. Science. 2017; 358(6362):522-8.)

SUMMARY OF THE INVENTION

The present invention is directed to compounds of Formula (I):

wherein R¹, R², R³, R⁴, R⁵, R⁶ and R⁹ are described below.

Also described herein are methods of treatment of Plasmodium infections comprising administering to a subject in need thereof a compound of Formula (I), or a pharmaceutically acceptable salt thereof. Also described herein are methods of treatment of Plasmodium infections comprising administering to a subject in need thereof a compound of Formula (I), or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.

Also described herein are methods of treating of malaria comprising administering to a subject in need thereof a compound of Formula (I), or a pharmaceutically acceptable salt thereof.

The present invention further provides the use of compositions, including pharmaceutical compositions, comprising one or more compounds of the invention (e.g., one compound of the invention), or a tautomer thereof, or a pharmaceutically acceptable salt or solvate of said compound(s) and/or said tautomer(s), optionally together with one or more additional therapeutic agents, optionally in an acceptable (e.g., pharmaceutically acceptable) carrier or diluent, for the treatment of malaria.

Moreover, the present invention provides methods for the use of pharmaceutical compositions comprising one or more of said compounds in the free form or in pharmaceutically acceptable salt form, together with one or more customary pharmaceutical excipient(s), for the treatment of Plasmodium infections, the treatment of malaria, the inhibition of plasmepsin X, or, possibly in some embodiments, the dual inhibition of plasmepsin X and plasmepsin IX. Methods for the use of combinations of the compounds or salts of the invention together with one or more additional pharmaceutically active agents are also provided.

The present invention may further provide methods for the inhibition of plasmepsin X, or dual inhibition of plasmepsin X and plasmepsin IX activity and for treatment, prevention, amelioration and/or delaying onset of diseases or disorders in which the inhibition of plasmepsin X and/or plasmepsin IX has or may have a therapeutic effect, e.g. malaria.

The present invention further provides methods for the inhibition of P. falciparum aspartic acid proteases. The present invention may further provide methods for blocking P. falciparum growth by inhibiting Plasmepsin X. The present invention may further provide methods for blocking P. falciparum growth by inhibiting both Plasmepsin X and Plasmepsin IX. In certain embodiments, the present invention may provide a method for blocking P. falciparum growth in an animal, preferably a human, comprising administering to the animal an inhibitor of Plasmepsin IX and Plasmepsin X or a pharmaceutically acceptable salt thereof.

The present invention may further provide methods for the treatment of malaria by inhibiting plasmepsin X. The present invention may further provide methods for the treatment of malaria by inhibiting both Plasmepsin X and Plasmepsin IX. In certain embodiments, the present invention may provide a method for treating malaria in an animal, preferably a human, comprising administering to the animal an inhibitor of Plasmepsin IX and Plasmepsin X or a pharmaceutically acceptable salt thereof.

These and other embodiments of the invention, which are described in detail below or will become clear to those of ordinary skill in the art, are included within the scope of the invention.

DETAILED DESCRIPTION OF THE INVENTION

Described herein are compounds having the structural Formula (I):

or a pharmaceutically acceptable salt thereof, wherein:

R¹ is a heterocycloalkyl, C₃-C₁₂cycloalkyl, aryl, C₁-C₆alkylaryl, wherein the heterocycloalkyl, C₃-C₁₂cycloalkyl, aryl or C₁-C₆alkylaryl is unsubstituted or substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, oxo, COOC₁-C₆alkyl, C₃-C₆cycloalkyl, spiroC₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) and C₁-C₆alkylN(R⁷)(R⁸);

R² is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH;

R³ is hydrogen, halogen, CN, OH, C₃-C₆cycloalkyl or C₁-C₆alkyl;

R⁴ is hydrogen, halogen, CN, OH, C₃-C₆cycloalkyl or C₁-C₆alkyl;

R⁵ is hydrogen, halogen, CN, OH, alkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) or C₁-C₆alkylN(R⁷)(R⁸) or when taken with R⁶ forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl;

R⁶ is hydrogen, halogen, CN, OH, alkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) or C₁-C₆alkylN(R⁷)(R⁸) or when taken with R⁵ forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl;

R⁷ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH;

R⁸ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH; and

R⁹ is hydrogen, halogen, CN, alkoxy, C₁-C₆alkyl, heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl, aryl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸), wherein the C₁-C₆alkyl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl, aryl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸), and wherein the heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl and aryl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, C₁-C₆alkyl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸).

In the embodiments described herein, R¹ is a heterocycloalkyl, C₃-C₁₂cycloalkyl, aryl, C₁-C₆alkylaryl, wherein the heterocycloalkyl, C₃-C₁₂cycloalkyl, aryl or C₁-C₆alkylaryl is unsubstituted or substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, oxo, COOC₁-C₆alkyl, C₃-C₆cycloalkyl, spiroC₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) and C₁-C₆alkylN(R⁷)(R⁸).

In certain embodiments, R¹ is a bicyclic ring. In certain embodiments, R¹ is a bicyclic heterocycloalkyl, bicyclic C₃-C₁₂cycloalkyl or bicyclic aryl ring. In certain embodiments, R¹ is a fused bycyclic ring having an A ring fused with a B ring, wherein the A ring is a 5, 6 or 7-membered saturated ring and the B ring is a phenyl or a 5 or 6-membered heteroaryl ring. In certain embodiments, the A ring includes at least one O, N, or S. In certain embodiments, the A ring includes at least one 0.

In certain embodiments, R¹ is a heterocycloalkyl. Non-limiting examples of monocyclic heterocycloalkyl groups include piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. Non-limiting examples of bicyclic heterocycloalkyl groups include, but are not limited to,

In certain embodiments, R¹ is a C₃-C₂cycloalkyl. In certain embodiments, the cycloalkyl is a monocyclic cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, the cycloalkyl is a bicyclic cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to:

In certain embodiments, R¹ is an aryl ring. Suitable examples of aryls include, but are not limited to, monocyclic aryl groups such as, phenyl and bicyclic aryl groups such as naphthyl.

In certain embodiments, R¹ is a C₁-C₆alkylaryl ring. Suitable examples of C₁-C₆alkylaryls include, but are not limited to:

In certain embodiments, R¹ is a chomane or indane.

In certain embodiments, R¹ is unsubstituted. In other embodiments, R¹ is substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, oxo, COOC₁-C₆alkyl, C₃-C₆cycloalkyl, spiroC₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) and C₁-C₆alkylN(R⁷)(R⁸). In certain embodiments, R¹ is substituted with 1 substituent. In certain embodiments, R¹ is substituted with 2 substituents. In certain embodiments, R¹ is substituted with 3 substituents. In certain embodiments, R¹ is substituted with 4 substituents. In certain embodiments, R¹ is substituted with 5 substituents.

In certain embodiments, R¹ is substituted with halogen. Examples of suitable halogens include, but are not limited to, chlorine, bromine, fluorine and iodine. In certain embodiments, R¹ is substituted with CN. In certain embodiments, R¹ is substituted with OH. In certain embodiments, R¹ is substituted with an oxo group.

In certain embodiments, R¹ is substituted with alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R¹ is substituted with C₁-C₆alkylOC₁-C₆alkyl. In certain embodiments, R¹ is substituted with haloalkoxy. Suitable haloalkoxys include, but are not limited to, trifluoromethoxy, fluoromethoxy and difluoromethoxy. In certain embodiments, R¹ is substituted with C₁-C₆alkylOC₁-C₆alkyl. Suitable C₁-C₆alkylOC₁-C₆alkyls include, but are not limited to, CH₂OCH₃. In certain embodiments, R¹ is substituted with C₁-C₆alkylCOOH. In certain embodiments, R¹ is substituted with COOH. In certain embodiments, R¹ is substituted with C₁-C₆alkylCOOC₁-C₆alkyl.

In certain embodiments, R¹ is substituted with C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R¹ is substituted with cyclopropyl:

In certain embodiments, R¹ is substituted with spiroC₃-C₆cycloalkyl. Suitable examples of spirocycloalkyls include, but are not limited to, spirocyclopropyl, spirocyclobutyl, spirocyclopentyl and spirocyclohexyl. In certain embodiments, R¹ is substituted with spirocyclobutyl:

In certain embodiments, R¹ is substituted with C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R¹ is substituted with haloC₁-C₆alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R¹ is substituted with C₁-C₆alkylOH. Examples of suitable alcohols, include, but are not limited to, methanol, ethanol, propanol, butanol and iso-butanol. In certain embodiments, R¹ is substituted with CON(R⁷)(R⁸). In certain embodiments, R¹ is substituted with N(R⁷)(R⁸). In certain embodiments, R¹ is substituted with C₁-C₆alkylN(R⁷)(R⁸), wherein R⁷ and R⁸ will be described in detail below.

In certain embodiments, R¹ is substituted with 1 to 4 substituents selected independently from the group consisting of bromine, fluorine, chlorine, methyl, ethyl, t-butyl, methoxy, OH, CN oxo, CH₂OCH₃, cyclopropyl, spirocyclobutyl, trifluoromethoxy and trifluoromethyl.

In certain embodiments, R¹ is

and is substituted with 1 to 4 substituents selected independently from the group consisting of bromine, fluorine, chlorine, methyl, ethyl, t-butyl, methoxy, OH, CN oxo, CH₂OCH₃, cyclopropyl, spirocyclobutyl, trifluoromethoxy and trifluoromethyl.

In certain embodiments, R¹ is

and is substituted with 1 to 4 substituents selected independently from the group consisting of bromine, fluorine, chlorine, methyl, ethyl, t-butyl, methoxy, OH, CN oxo, CH₂OCH₃, cyclopropyl, spirocyclobutyl, trifluoromethoxy and trifluoromethyl.

In certain embodiments, R¹ is

and is substituted with 1 to 4 substituents selected independently from the group consisting of bromine, fluorine, chlorine, methyl, ethyl, t-butyl, methoxy, OH, CN oxo, CH₂OCH₃, cyclopropyl, spirocyclobutyl, trifluoromethoxy and trifluoromethyl. In certain embodiments described herein, R² is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH. In certain embodiments, R² is hydrogen. In certain embodiments, R² is C₁-C₆alkylCOOH. In certain embodiments, R² is COOH. In certain embodiments, R² is C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R² is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl.

In certain embodiments, R² is haloC₁-C₆alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R² is C₁-C₆alkylOH. Examples of suitable alcohols, include, but are not limited to, methanol, ethanol, propanol, butanol and iso-butanol.

With regard to the compounds described herein, R³ is hydrogen, halogen, CN, OH, C₃-C₆cycloalkyl or C₁-C₆alkyl. In certain embodiments, R³ is hydrogen. In certain embodiments, R³ is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R³ is CN. In certain embodiments, R³ is OH.

In certain embodiments, R³ is C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R³ is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl.

In certain embodiments, R³ is hydrogen, fluorine, methyl, ethyl or

In certain embodiments, R³ is taken with R⁴ to form oxetanyl.

With regard to the compounds described herein, R⁴ is hydrogen, halogen, CN, OH, C₃-C₆cycloalkyl or C₁-C₆alkyl. In certain embodiments, R⁴ is hydrogen. In certain embodiments, R⁴ is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R⁴ is CN. In certain embodiments, R⁴ is OH.

In certain embodiments, R⁴ is C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R⁴ is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl.

In certain embodiments, R⁴ is hydrogen, methyl, or fluorine. In certain embodiments, R³ and R⁴ are both hydrogen, methyl or fluorine.

In certain embodiments, R³ is hydrogen and R⁴ is hydrogen, methyl or fluorine.

With regard to the compounds described herein, R⁵ is hydrogen, halogen, CN, OH, alkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) or C₁-C₆alkylN(R⁷)(R⁸) or when taken with R⁶ forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl. In certain embodiments, R⁵ is hydrogen. In certain embodiments, R⁵ is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R⁵ is CN. In certain embodiments, R⁵ is OH.

In certain embodiments, R⁵ is alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R⁵ is C₁-C₆alkylOC₁-C₆alkyl. In certain embodiments, R⁵ is COOH. In certain embodiments, R⁵ is C₁-C₆alkylCOOH. In certain embodiments, R⁵ is C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R⁵ is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R⁵ is haloC₁-C₆alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R⁵ is C₁-C₆alkylOH. Examples of suitable alcohols include, but are not limited to, methanol, ethanol, propanol, butanol and iso-butanol. In certain embodiments, R⁵ is CON(R⁷)(R⁸). In certain embodiments, R⁵ is N(R⁷)(R⁸). In certain embodiments, R⁵ is C₁-C₆alkylN(R⁷)(R⁸). R⁷ and R⁸ will be discussed in detail below.

In certain embodiments, R⁵ is taken with R⁶ and forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl. In certain embodiments, R⁵ is taken with R⁶ and forms a C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R⁵ is taken with R⁶ and forms a C₃-C₆heterocycloalkyl. Suitable examples of heterocycloalkyls include, but are not limited to, piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof.

In certain embodiments, R⁵ is methyl, ethyl or t-butyl.

With regard to the compounds described herein, R⁶ is hydrogen, halogen, CN, OH, alkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) or C₁-C₆alkylN(R⁷)(R⁸) or when taken with R⁵ forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl. In certain embodiments, R⁶ is hydrogen. In certain embodiments, R⁶ is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R⁶ is CN. In certain embodiments, R⁶ is OH.

In certain embodiments, R⁶ is alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R⁶ is C₁-C₆alkylOC₁-C₆alkyl. In certain embodiments, R⁶ is COOH. In certain embodiments, R⁶ is C₁-C₆alkylCOOH. In certain embodiments, R⁶ is C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R⁶ is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R⁶ is haloC₁-C₆alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R⁶ is C₁-C₆alkylOH. Examples of suitable alcohols include, but are not limited to, methanol, ethanol, propanol, butanol and iso-butanol. In certain embodiments, R⁶ is CON(R⁷)(R⁸). In certain embodiments, R⁶ is N(R⁷)(R⁸). In certain embodiments, R⁶ is C₁-C₆alkylN(R⁷)(R⁸). R⁷ and R⁸ will be discussed in detail below.

In certain embodiments, R⁶ is taken with R⁵ and forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl. In certain embodiments, R⁶ is taken with R⁵ and forms a C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R⁶ is taken with R⁵ and forms a C₃-C₆heterocycloalkyl. Suitable examples of heterocycloalkyls include, but are not limited to, piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof.

In certain embodiments, R⁶ is methyl, ethyl or t-butyl.

With regard to the compounds described herein, R⁷ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH.

In certain embodiments, R⁷ is hydrogen. In certain embodiments, R⁷ is C₁-C₆alkylCOOH. In certain embodiments, R⁷ is COOH. In certain embodiments, R⁷ is C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R⁷ is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R⁷ is haloC₁-C₆alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R⁷ is C₁-C₆alkylOH. Examples of suitable alcohols include, but are not limited to, methanol, ethanol, propanol, butanol and iso-butanol.

With regard to the compounds described herein, R⁸ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH.

In certain embodiments, R⁸ is hydrogen. In certain embodiments, R⁸ is C₁-C₆alkylCOOH. In certain embodiments, R⁸ is COOH. In certain embodiments, R⁸ is C₃-C₆cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, R⁸ is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R⁸ is haloC₁-C₆alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R⁸ is C₁-C₆alkylOH. Examples of suitable alcohols include, but are not limited to, methanol, ethanol, propanol, butanol and iso-butanol.

With regard to the compounds described herein, R⁹ is hydrogen, halogen, CN, OH, alkoxy, C₁-C₆alkyl, heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl, aryl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸), wherein the C₁-C₆alkyl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl, aryl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸), and wherein the heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl and aryl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, C₁-C₆alkyl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸).

In certain embodiments, R⁹ is hydrogen. In certain embodiments, R⁹ is halogen. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, or iodine. In certain embodiments, R⁹ is CN.

In certain embodiments, R⁹ is alkoxy. Suitable alkoxys include, but are not limited to, methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. In certain embodiments, R⁹ is C₁-C₆alkyl. Examples of C₁-C₆alkyl groups can include but are not limited to, methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, 1-methylbutyl, 2-methylbutyl, 1,2-dimethylpropyl, 1-ethylpropyl, n-hexyl, isohexyl, 1-methylpentyl, 2-methylpentyl, 3-methylpentyl, 1,1-dimethylbutyl, 1,2-dimethylbutyl, 2,2-dimethylbutyl, 1-ethylbutyl, 1,1,2-trimethylpropyl, 1,2,2-trimethylpropyl, 1-ethyl-2-methylpropyl and 1-ethyl-1-methylpropyl. In certain embodiments, R⁹ is haloC₁-C₆alkyl. Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl.

In certain embodiments, R⁹ is a heterocycloalkyl. Non-limiting examples of monocyclic heterocycloalkyl groups include piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. In certain embodiments, R⁹ is a heteroaryl group. Suitable heteroaryls include, but are not limited to, pyridyl (pyridinyl), imidazolyl, triazolyl, triazinyl, pyrimidyl, pyridazinyl, indolizinyl, cinnolinyl, phthalazinyl, quinazolinyl, naphthyridinyl, quinoxalinyl, purinyl, benzimidazolyl, quinolyl, isoquinolyl. In certain embodiments, R⁹ is a pyridine.

In certain embodiments, R⁹ is a C₃-C₁₂cycloalkyl. In certain embodiments, the cycloalkyl is a monocyclic cycloalkyl. Suitable examples of cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl. In certain embodiments, the cycloalkyl is a bicyclic cycloalkyl.

In certain embodiments, R⁹ is an aryl ring. Suitable examples of aryls include, but are not limited to, monocyclic aryl groups such as, phenyl and bicyclic aryl groups such as naphthyl.

In certain embodiments, R⁹ is COOH. In certain embodiments, R⁹ is oxo. In certain embodiments, R⁹ is C₁-C₆alkylCOOC₁-C₆alkyl. In certain embodiments, R⁹ is haloC₁-C₆alkyl.

Suitable examples of haloalkyls include, but are not limited to, fluoromethyl, difluoromethyl, trifluoromethyl, 2-fluoroethyl, 1,2-difluoroethyl and 2,2-difluoroethyl. In certain embodiments, R⁹ is CON(R⁷)(R⁸). In certain embodiments, R⁹ is N(R⁷)(R⁸). In certain embodiments, R⁹ is C₁-C₆alkylN(R⁷)(R⁸).

In certain embodiments, R⁹ is unsubstituted or substituted. In certain embodiments, when R⁹ is C₁-C₆alkyl, C₁-C₆alkyl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl, aryl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸). In certain embodiments, R⁹ is

In certain embodiments, R⁹ is

In certain embodiments, when R⁹ is heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl or aryl, the heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl or aryl the can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, C₁-C₆alkyl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸). In certain embodiments, R⁹ is pyridine substituted with fluorine, chlorine or methoxy. In certain embodiments, R⁹ is phenyl substituted with CN.

Also described herein are compounds of Formula (IA), (IB), (IC) and (ID):

wherein R³, R⁴, R⁵, R⁶ and R⁹ are as described above;

R¹⁰ is halogen, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₃-C₆cycloalkyl, spiroC₃-C₆cycloalkyl, C₁-C₆alkyl or haloC₁-C₆alkyl;

R¹¹ is OH or C₁-C₆alkyl;

R¹² is C₁-C₆alkyl; and

m, n and q are independently 0, 1, 2, 3 or 4.

In each of the various embodiments of the invention, in the compounds used in the methods herein, each variable (including those in each of Formula (I), (IA), (IB), (IC) and (ID), and the various embodiments thereof) it shall be understood that each variable is to be selected independently of the others unless otherwise indicated.

In each of the various embodiments of the invention, the compounds described herein, including those in each of Formula (I), (IA), (IB), (IC) and (ID) and the various embodiments thereof, may exit in different forms of the compounds such as, for example, any solvates, hydrates, stereoisomers, and tautomers of said compounds and of any pharmaceutically acceptable salts thereof.

In certain embodiments, compounds described herein include:

The compounds described and exemplified herein, can also be described using IUPAC nomenclature:

-   [1-[1-[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[1-[(1R,2R)-2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-6-oxo-1-[[(1S,2S)-2-[[(1S)-1-phenylethyl]carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-6-oxo-1-[[2-[[(1R)-1-phenylethyl]carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1S,2S)-2-[[(4S)-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1S,2S)-2-[[(4R)-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2-[[(4S)-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[(1S,2S)-2-[[(1R,2R)-2-hydroxyindan-1-yl]carbamoyl]cyclopropyl]methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-6-oxo-1-[[(1R,2R)-2-[[2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   (1R,2R)—N-[(4S)-chroman-4-yl]-2-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-(3-pyridyl)methyl]cyclopropanecarboxamide; -   (1R,2R)-2-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-(3-pyridyl)methyl]-N-[(1R,2R)-2-hydroxyindan-1-yl]cyclopropanecarboxamide; -   [4,4-diethyl-1-[[(1R,2R)-2-[(3-hydroxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-6-oxo-1-[pyridin-1-ium-3-yl-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[1-[(1R,2R)-2-[[(1R,2R)-2-hydroxyindan-1-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[3-methoxy-1-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[3-[[(4S)-chroman-4-yl]carbamoyl]-2,2-difluoro-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[3-[[(4R)-chroman-4-yl]carbamoyl]-2,2-difluoro-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[3-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]-2,2-difluoro-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[3-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]-2,2-difluoro-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-difluoro-3-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-difluoro-3-[[(2R,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-difluoro-3-[[(3S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-difluoro-3-[[(3S,4S)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[1-[(1R,2R)-2-[(3,3-dimethylchroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-1-[1-[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-isopropyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-isopropyl-1-[3-methoxy-1-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-4-isopropyl-1-[3-methoxy-1-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[1-[(1R,2R)-2-[[(4S)-chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-isopropyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-3-methoxy-1-[(1R,2R)-2-[[(2S,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[3-methoxy-1-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[3-methoxy-1-[(1R,2R)-2-[[(2R,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]-3-methyl-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]-3-methyl-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(3     S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[2-methyl-3-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[2-methyl-3-[[(2R,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   2-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)methyl]-N-[(1R,2S)-2-hydroxy-2-methyl-indan-1-yl]-3-methyl-cyclopropanecarboxamide; -   2-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)methyl]-N-[(1R,2R)-2-hydroxy-2-methyl-indan-1-yl]-3-methyl-cyclopropanecarboxamide; -   2-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)methyl]-N-[(1S,2S)-2-hydroxy-2-methyl-indan-1-yl]-3-methyl-cyclopropanecarboxamide; -   [4,4-diethyl-1-[[2-[[(3S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]-3-methyl-cyclopropyl]methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[2-[[(3R,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]-3-methyl-cyclopropyl]methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-dimethyl-3-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   3-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)methyl]-2,2-dimethyl-N-[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]cyclopropanecarboxamide; -   [4,4-dimethyl-6-oxo-1-[pyridin-1-ium-3-yl-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[2-[[(1R,2R)-2-hydroxyindan-1-yl]carbamoyl]-3-methyl-cyclopropyl]methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   3-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)methyl]-N-[(4S)-2,2-dimethylchroman-4-yl]-2,2-dimethyl-cyclopropanecarboxamide; -   2-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)methyl]-N-[(3R,4R)-3-hydroxy-3-methyl-chroman-4-yl]-3-methyl-cyclopropanecarboxamide; -   2-[(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)methyl]-N-[(3S,4R)-3-hydroxy-3-methyl-chroman-4-yl]-3-methyl-cyclopropanecarboxamide; -   [1-[[(2S)-[(2,2-dimethyl-3H-benzofuran-3-yl)carbamoyl]-3-methyl-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(2R)-[(2,2-dimethyl-3H-benzofuran-3-yl)carbamoyl]-3-methyl-cyclopropyl]methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(6-methoxy-3-pyridyl)-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-(6-methoxy-3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(3     S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]-(6-methoxy-3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-(5-methoxy-3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(5-methoxy-3-pyridyl)-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(3S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]-(5-methoxy-3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-dimethyl-3-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-dimethyl-3-[[(2R,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-dimethyl-3-[[(2S,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2,2-dimethyl-3-[[(2S,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(4-cyanophenyl)-[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(4-cyanophenyl)-[(1R,2R)-2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(4-cyanophenyl)-[(1R,2R)-2-[[(3S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(4-cyanophenyl)-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(4-cyanophenyl)-[(1R,2R)-2-[[(2R,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[3-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]-2,2-dimethyl-cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[3-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]-2,2-dimethyl-cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(3-cyanophenyl)-[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(3-cyanophenyl)-[(1R,2R)-2-[[(3     S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(3-cyanophenyl)-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(3-cyanophenyl)-[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-dimethyl-1-[[2-methyl-3-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[2-methyl-3-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-diethyl-1-[[2-methyl-3-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]-3-methyl-cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]-3-methyl-cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-diethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(5-chloro-3-pyridyl)-[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(5-chloro-3-pyridyl)-[(1R,2R)-2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(5-chloro-3-pyridyl)-[(1R,2R)-2-[[(3     S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(5-chloro-3-pyridyl)-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(5-chloro-3-pyridyl)-[(1R,2R)-2-[[(2R,4R)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[1-[(1R,2R)-2-[[(3S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[3-methoxy-1-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[3-methoxy-1-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[1-[(1R,2R)-2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-1-[1-[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(7-methoxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[(5-fluoro-3-pyridyl)-[(1R,2R)-2-[[(3     S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-1-[[(1R,2R)-2-[[(4S)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-(5-fluoro-3-pyridyl)methyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[[(1R,2R)-2-[[(4R)-2,2-dimethylchroman-4-yl]carbamoyl]cyclopropyl]-(5-fluoro-3-pyridyl)methyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[(5-fluoro-3-pyridyl)-[(1R,2R)-2-[[(2R,4S)-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-4-ethyl-1-[[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-(5-fluoro-3-pyridyl)methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-(5-fluoro-3-pyridyl)methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[3-methoxy-1-[(1R,2R)-2-[(7-methoxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(5-methoxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-3-methoxy-1-[(1R,2R)-2-[(5-methoxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-3-methoxy-1-[(1R,2R)-2-[(5-methoxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(2-ethyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(2-ethyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(7-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(7-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(7-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(7-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(2-ethyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(2-isopropyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(2-isopropyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(2-isopropyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(2-isopropyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(2-isopropyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(2-isopropyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-3-methoxy-1-[(1R,2R)-2-[[2-(methoxymethyl)-2-methyl-chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-3-methoxy-1-[(1R,2R)-2-[[2-(methoxymethyl)-2-methyl-chroman-4-yl]carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-3-methoxy-1-[(1R,2R)-2-[(2,2,7-trimethylchroman-4-yl)carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-dimethyl-6-oxo-1-[[(1R,2R)-2-[(2,2,7-trimethylchroman-4-yl)carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-dimethyl-6-oxo-1-[(S)-pyridin-1-ium-3-yl-[(1R,2R)-2-[(2,2,7-trimethylchroman-4-yl)carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-dimethyl-6-oxo-1-[(R)-pyridin-1-ium-3-yl-[(1R,2R)-2-[(2,2,7-trimethylchroman-4-yl)carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(5-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(5-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(5-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(5-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(5-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(7-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(7-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(7-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(7-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(2,2-diethylchroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(2,2-diethylchroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(8-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(8-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(6,8-dichloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(6,8-dichloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[(4S)-6-chloro-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(5-methoxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(5-methoxy-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(6,8-dichloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[5-fluoro-2,2-dimethyl-8-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[(4S)-6-chloro-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[[(1R,2R)-2-[(3,3-dimethylchroman-4-yl)carbamoyl]cyclopropyl]-(5-fluoro-3-pyridyl)methyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-1-[[(1R,2R)-2-[(3,3-dimethylchroman-4-yl)carbamoyl]cyclopropyl]-(5-fluoro-3-pyridyl)methyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[(5-fluoro-3-pyridyl)-[(1R,2R)-2-[(3-hydroxy-2,2,3-trimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-4-ethyl-1-[(5-fluoro-3-pyridyl)-[(1R,2R)-2-[(3-hydroxy-2,2,3-trimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[(5-fluoro-3-pyridyl)-[(1R,2R)-2-[[3-hydroxy-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-4-ethyl-1-[(5-fluoro-3-pyridyl)-[(1R,2R)-2-[[3-hydroxy-2-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(2-ethyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(2-ethyl-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(6,8-dichloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(6,8-dichloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-5-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[[2,2-dimethyl-5-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[[2-(methoxymethyl)-2-methyl-chroman-4-yl]carbamoyl]cyclopropyl]-(3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[[2-(methoxymethyl)-2-methyl-chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[[2-(methoxymethyl)-2-methyl-chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[[2-(methoxymethyl)-2-methyl-chroman-4-yl]carbamoyl]cyclopropyl]-(3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(8-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(8-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(8-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(8-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(6,8-difluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   30     [1-[(1S)-1-[(1R,2R)-2-[(6,8-difluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[5-fluoro-2,2-dimethyl-8-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[[5-fluoro-2,2-dimethyl-8-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[[5-fluoro-2,2-dimethyl-8-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[[5-fluoro-2,2-dimethyl-8-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(5-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(5-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(5-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-(3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(5-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-(3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[2,2-dimethyl-5-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   (1R,2R)—N-(5-chloro-2,2-dimethyl-chroman-4-yl)-2-[(2-imino-4,4-dimethyl-6-oxo-hexahydropyrimidin-1-yl)methyl]cyclopropanecarboxamide; -   [1-[(S)-[(1R,2R)-2-[[(4S)-6-chloro-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(8-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(8-chloro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-(3-pyridyl)methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(S)-[(1R,2R)-2-[(6,8-difluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(R)-[(1R,2R)-2-[(6,8-difluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-pyridin-1-ium-3-yl-methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[(1R)-3-methoxy-1-[(1R,2R)-2-[[(4S)-2,2,6-trimethylchroman-4-yl]carbamoyl]cyclopropyl]propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[(1R)-1-[(1R,2R)-2-[[(4S)-6-chloro-2,2-dimethyl-chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethyl)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-dimethyl-6-oxo-1-[[(1R,2R)-2-[[(4S)-2,2,6-trimethylchroman-4-yl]carbamoyl]cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-6-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-ethyl-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4R)-4-ethyl-1-[(1R)-1-[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [(4S)-4-ethyl-1-[(1R)-1-[(1R,2R)-2-[(6-fluoro-2,2-dimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4-methyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-3-methoxy-1-[(1R,2R)-2-(spiro[chromane-2,1′-cyclobutane]-4-ylcarbamoyl)cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   10     [1-[(1S)-3-methoxy-1-[(1R,2R)-2-(spiro[chromane-2,1′-cyclobutane]-4-ylcarbamoyl)cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-3-methoxy-1-[(1R,2R)-2-[[(4S)-2,2,6-trimethylchroman-4-yl]carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(2-ethylchroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[[2,2-dimethyl-7-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[[2,2-dimethyl-7-(trifluoromethoxy)chroman-4-yl]carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [4,4-dimethyl-6-oxo-1-[[(1R,2R)-2-(spiro[chromane-2,1′-cyclobutane]-4-ylcarbamoyl)cyclopropyl]methyl]hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(6-chloro-2,2,7-trimethyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-1-[(1R,2R)-2-[(6-chloro-2,2,7-trimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-1-[(1R,2R)-2-[(6-chloro-2,2,7-trimethyl-chroman-4-yl)carbamoyl]cyclopropyl]-3-methoxy-propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1R)-3-methoxy-1-[(1R,2R)-2-[(2,2,6,7-tetramethylchroman-4-yl)carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[(1S)-3-methoxy-1-[(1R,2R)-2-[(2,2,6,7-tetramethylchroman-4-yl)carbamoyl]cyclopropyl]propyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(6-fluoro-2-methyl-chroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   [1-[[(1R,2R)-2-[(2-cyclopropylchroman-4-yl)carbamoyl]cyclopropyl]methyl]-4,4-dimethyl-6-oxo-hexahydropyrimidin-2-ylidene]ammonium;2,2,2-trifluoroacetate; -   (1R,2R)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]-N-[(4R)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-butyl]-N-[(4S)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-butyl]-N-[(4S)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-butyl]-N-[(4S)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-butyl]-N-[(4S)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-butyl]-N-[(4R)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-butyl]-N-[(4R)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-butyl]-N-[(4R)-2,2-dimethylchroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]-N-[(3R,4S)-3-hydroxy-2,2-dimethyl-chroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]-N-[(3S,4R)-3-hydroxy-2,2-dimethyl-chroman-4-yl]cyclopropanecarboxamide; -   (1R,2R)—N-[(4S)-6-chloro-2,2-dimethyl-chroman-4-yl]-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide; -   (1R,2R)—N-(7-chloro-2,2-dimethyl-chroman-4-yl)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide; -   (1R,2R)—N-(7-chloro-2,2-dimethyl-chroman-4-yl)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide; -   (1R,2R)—N-(8-chloro-2,2-dimethyl-chroman-4-yl)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide; -   (1R,2R)—N-(8-chloro-2,2-dimethyl-chroman-4-yl)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide; -   (1R,2R)—N-(5-chloro-2,2-dimethyl-chroman-4-yl)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide; -   (1R,2R)—N-(5-chloro-2,2-dimethyl-chroman-4-yl)-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide;     and -   (1R,2R)—N-[(3S,4R)-6-chloro-3-hydroxy-2,2-dimethyl-chroman-4-yl]-2-[(1R)-1-(4,4-diethyl-2-imino-6-oxo-hexahydropyrimidin-1-yl)-3-methoxy-propyl]cyclopropanecarboxamide.

Definitions and Abbreviations

The terms used herein have their ordinary meaning and the meaning of such terms is independent at each occurrence thereof. That notwithstanding and except where stated otherwise, the following definitions apply throughout the specification and claims. Chemical names, common names and chemical structures may be used interchangeably to describe that same structure. These definitions apply regardless of whether a term is used by itself or in combination with other terms, unless otherwise indicated. Hence the definition of “alkyl” applies to “alkyl” as well as the “alkyl” portion of “hydroxyalkyl”, “haloalkyl”, arylalkyl-, alkylaryl-, “alkoxy” etc.

It shall be understood that, in the various embodiments of the invention described herein, any variable not explicitly defined in the context of the embodiment is as defined in Formula (I).

In the various embodiments described herein, each variable is selected independently of the others unless otherwise indicated.

“Drug resistant” means, in connection with a Plasmodium parasite strain, a Plasmodium species which is no longer susceptible to at least one previously effective drug; which has developed the ability to withstand attack by at least one previously effective drug. A drug resistant strain may relay that ability to withstand to its progeny. Said resistance may be due to random genetic mutations in the bacterial cell that alters its sensitivity to a single drug or to different drugs.

“Patient” includes both human and non-human animals. Non-human animals include those research animals and companion animals such as mice, rats, primates, monkeys, chimpanzees, great apes, dogs, and house cats.

“Pharmaceutical composition” (or “pharmaceutically acceptable composition”) means a composition suitable for administration to a patient. Such compositions may contain the neat compound (or compounds) of the invention or mixtures thereof, or salts, solvates, prodrugs, isomers, or tautomers thereof, and one or more pharmaceutically acceptable carriers or diluents. The term “pharmaceutical composition” is also intended to encompass both the bulk composition and individual dosage units comprised of one or more (e.g., two) pharmaceutically active agents such as, for example, a compound of the present invention and an additional agent selected from the lists of the additional agents described herein, along with any pharmaceutically inactive excipients. The bulk composition and each individual dosage unit can contain fixed amounts of the afore-said “more than one pharmaceutically active agents”. The bulk composition is material that has not yet been formed into individual dosage units. An illustrative dosage unit is an oral dosage unit such as tablets, pills and the like. Similarly, the herein-described method of treating a patient by administering a pharmaceutical composition of the present invention is also intended to encompass the administration of the afore-said bulk composition and individual dosage units.

“Halogen” and “halo” mean fluorine, chlorine, bromine, or iodine. Preferred are fluorine, chlorine and bromine.

“Alkyl” means an aliphatic hydrocarbon group which may be straight or branched and comprising about 1 to about 20 carbon atoms in the chain. Preferred alkyl groups contain about 1 to about 12 carbon atoms in the chain. More preferred alkyl groups contain about 1 to about 6 carbon atoms in the chain. Branched means that one or more lower alkyl groups such as methyl, ethyl or propyl, are attached to a linear alkyl chain. “Lower alkyl” means a group having about 1 to about 6 carbon atoms in the chain which may be straight or branched. Non-limiting examples of suitable alkyl groups include methyl, ethyl, n-propyl, isopropyl and t-butyl.

“Haloalkyl” means an alkyl as defined above wherein one or more hydrogen atoms on the alkyl is replaced by a halo group defined above.

“Aryl” means an aromatic monocyclic or multicyclic ring system comprising about 6 to about 14 carbon atoms, preferably about 6 to about 10 carbon atoms. The aryl group can be optionally substituted with one or more “ring system substituents” which may be the same or different, and are as defined herein. Non-limiting examples of suitable aryl groups include phenyl and naphthyl. “Monocyclic aryl” means phenyl.

“Cycloalkyl” means a non-aromatic mono- or multicyclic ring system comprising about 3 to about 12 carbon atoms, preferably about 3 to about 10 carbon atoms. Preferred cycloalkyl rings contain about 5 to about 10 ring atoms. The cycloalkyl can be optionally substituted with one or more substituents, which may be the same or different, as described herein. Monocyclic cycloalkyl refers to monocyclic versions of the cycloalkyl moieties described herein. Non-limiting examples of suitable monocyclic cycloalkyls include cyclopropyl, cyclopentyl, cyclohexyl, cycloheptyl and the like. Multicyclic cycloalkyls refers to multicyclic, including bicyclic, rings that include a non-aromatic ring. Non-limiting examples of suitable multicyclic cycloalkyls include 1-decalinyl, norbornyl, adamantyl and the like. In certain embodiments, a non-aromatic ring is fused to an aromatic ring. Further non-limiting examples of cycloalkyl include the following:

“Heterocycloalkyl” (or “heterocyclyl”) means a non-aromatic, saturated or partially saturated monocyclic or multicyclic ring system comprising about 3 to about 10 ring atoms, preferably about 5 to about 10 ring atoms, in which one or more of the atoms in the ring system is an element other than carbon, for example nitrogen, oxygen or sulfur, alone or in combination. There are no adjacent oxygen and/or sulfur atoms present in the ring system. Preferred heterocyclyls contain about 5 to about 6 ring atoms. The prefix aza, oxa or thia before the heterocyclyl root name means that at least a nitrogen, oxygen or sulfur atom respectively is present as a ring atom. Any —NH in a heterocyclyl ring may exist protected such as, for example, as an —N(Boc), —N(CBz), —N(Tos) group and the like; such protections are also considered part of this invention. The heterocyclyl can be optionally substituted by one or more substituents, which may be the same or different, as described herein. The nitrogen or sulfur atom of the heterocyclyl can be optionally oxidized to the corresponding N-oxide, S-oxide or S,S-dioxide. Thus, the term “oxide,” when it appears in a definition of a variable in a general structure described herein, refers to the corresponding N-oxide, S-oxide, or S,S-dioxide. “Heterocyclyl” also includes rings wherein ═O replaces two available hydrogens on the same carbon atom (i.e., heterocyclyl includes rings having a carbonyl group in the ring). Such ═O groups may be referred to herein as “oxo.” An example of such a moiety is pyrrolidinone (or pyrrolidone):

As used herein, the term “monocyclic heterocycloalkyl” refers monocyclic versions of the heterocycloalkyl moieties described herein and include a 4- to 7-membered monocyclic heterocycloalkyl groups comprising from 1 to 4 ring heteroatoms, said ring heteroatoms being independently selected from the group consisting of N, N-oxide, O, S, S-oxide, S(O), and S(O)₂. The point of attachment to the parent moiety is to any available ring carbon or ring heteroatom. Non-limiting examples of monocyclic heterocycloalkyl groups include piperidyl, oxetanyl, pyrrolyl, piperazinyl, morpholinyl, thiomorpholinyl, thiazolidinyl, 1,4-dioxanyl, tetrahydrofuranyl, tetrahydrothiophenyl, beta lactam, gamma lactam, delta lactam, beta lactone, gamma lactone, delta lactone, and pyrrolidinone, and oxides thereof. A non-limiting example of a monocyclic heterocycloalkyl group include the moiety:

Non-limiting examples of multicyclic heterocycloalkyl groups include, bicyclic heterocycloalkyl groups. Specific examples include, but are not limited to,

The term “heteroaryl” means an aromatic heterocycle that contains at least one ring heteroatom selected from O, S and N. Examples of heteroaryl groups include pyridyl (pyridinyl), oxazolyl, imidazolyl, triazolyl, furyl, triazinyl, thienyl, pyrimidyl, pyridazinyl, indolizinyl, cinnolinyl, phthalazinyl, quinazolinyl, naphthyridinyl, quinoxalinyl, purinyl, benzimidazolyl, quinolyl, isoquinolyl, and the like.

“SpiroC₁-C₆cycloalkyl” means a cycloalkyl that is linked to another ring system by one common atom, for example

“Alkoxy” means an alkyl-O— group in which the alkyl group is as previously described. Non-limiting examples of suitable alkoxy groups include methoxy, ethoxy, n-propoxy, isopropoxy and n-butoxy. The bond to the parent moiety is through the ether oxygen.

The term “substituted” means that one or more hydrogens on the designated atom is replaced with a selection from the indicated group, provided that the designated atom's normal valency under the existing circumstances is not exceeded, and that the substitution results in a stable compound. Combinations of substituents and/or variables are permissible only if such combinations result in stable compounds. By “stable compound” or “stable structure” is meant a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent.

The term “optionally substituted” means optional substitution with the specified groups, radicals or moieties.

When a variable appears more than once in a group, e.g., R⁸ in —N(R⁸)₂, or a variable appears more than once in a structure presented herein, the variables can be the same or different.

A solid line

, as a bond generally indicates a mixture of, or either of, the possible isomers, e.g., containing (R)- and (S)-stereochemistry. For example:

means containing either one of or both N

The wavy line

, as used herein shown crossing a line representing a chemical bond, indicates a point of attachment to the rest of the compound. Lines drawn into the ring systems, such as, for example

indicates that the indicated line (bond) may be attached to any of the substitutable ring atoms.

“Oxo” is defined as an oxygen atom that is double bonded to a ring carbon in a cycloalkyl, cycloalkenyl, heterocycloalkyl, heterocycloalkenyl, or another ring described herein, e.g.,

In this specification, where there are multiple oxygen and/or sulfur atoms in a ring system, there cannot be any adjacent oxygen and/or sulfur present in said ring system.

As well known in the art, a bond drawn from a particular atom wherein no moiety is depicted at the terminal end of the bond indicates a methyl group bound through that bond to the atom, unless stated otherwise. For example:

In another embodiment, the compounds useful in the methods of the invention, and/or compositions comprising them useful in said methods, are present in isolated and/or purified form. The term “purified”, “in purified form” or “in isolated and purified form” for a compound refers to the physical state of said compound after being isolated from a synthetic process (e.g. from a reaction mixture), or natural source or combination thereof. Thus, the term “purified”, “in purified form” or “in isolated and purified form” for a compound refers to the physical state of said compound (or a tautomer or stereoisomer thereof, or pharmaceutically acceptable salt or solvate of said compound, said stereoisomer, or said tautomer) after being obtained from a purification process or processes described herein or well known to the skilled artisan (e.g., chromatography, recrystallization and the like), in sufficient purity to be suitable for in vivo or medicinal use and/or characterizable by standard analytical techniques described herein or well known to the skilled artisan.

It shall be understood that any carbon as well as heteroatom with unsatisfied valences in the text, schemes, examples and tables herein is assumed to have the sufficient number of hydrogen atom(s) to satisfy the valences.

When a functional group in a compound is termed “protected”, this means that the group is in modified form to preclude undesired side reactions at the protected site when the compound is subjected to a reaction. Suitable protecting groups will be recognized by those with ordinary skill in the art as well as by reference to standard textbooks such as, for example, T. W. Greene et al, Protective Groups in Organic Synthesis (1991), Wiley, New York.

Another embodiment provides prodrugs and/or solvates of the compounds of the invention. A discussion of prodrugs is provided in T. Higuchi and V. Stella, Pro-drugs as Novel Delivery Systems (1987) 14 of the A.C.S. Symposium Series, and in Bioreversible Carriers in Drug Design, (1987) Edward B. Roche, ed., American Pharmaceutical Association and Pergamon Press. The term “prodrug” means a compound (e.g., a drug precursor) that is transformed in vivo to yield a compound of the invention or a pharmaceutically acceptable salt, hydrate or solvate of the compound. The transformation may occur by various mechanisms (e.g., by metabolic or chemical processes), such as, for example, through hydrolysis in blood. A discussion of the use of prodrugs is provided by T. Higuchi and W. Stella, “Pro-drugs as Novel Delivery Systems,” Vol. 14 of the A.C.S. Symposium Series, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987.

For example, if a compound useful in the methods of the invention or a pharmaceutically acceptable salt thereof, contains a carboxylic acid functional group, a prodrug can comprise an ester formed by the replacement of the hydrogen atom of the acid group with a group such as, for example, (C₁-C₈)alkyl, (C₂-C₁₂)alkanoyloxymethyl, 1-(alkanoyloxy)ethyl having from 4 to 9 carbon atoms, 1-methyl-1-(alkanoyloxy)-ethyl having from 5 to 10 carbon atoms, alkoxycarbonyloxymethyl having from 3 to 6 carbon atoms, 1-(alkoxycarbonyloxy)ethyl having from 4 to 7 carbon atoms, 1-methyl-1-(alkoxycarbonyloxy)ethyl having from 5 to 8 carbon atoms, N-(alkoxycarbonyl)aminomethyl having from 3 to 9 carbon atoms, 1-(N-(alkoxycarbonyl)amino)ethyl having from 4 to 10 carbon atoms, 3-phthalidyl, 4-crotonolactonyl, gamma-butyrolacton-4-yl, di-N,N—(C₁-C₂)alkylamino(C₂-C₃)alkyl (such as β-dimethylaminoethyl), carbamoyl-(C₁-C₂)alkyl, N,N-di (C₁-C₂)alkylcarbamoyl-(C₁-C₂)alkyl and piperidino-, pyrrolidino- or morpholino(C₂-C₃)alkyl, and the like.

Similarly, if a compound used in the methods of the invention contains an alcohol functional group, a prodrug can be formed by the replacement of the hydrogen atom of the alcohol group with a group such as, for example, (C₁-C₆)alkanoyloxymethyl, 1-((C₁-C₆)alkanoyloxy)ethyl, 1-methyl-1-((C₁-C₆)alkanoyloxy)ethyl, (C₁-C₆)alkoxycarbonyloxymethyl, N—(C₁-C₆)alkoxycarbonylaminomethyl, succinoyl, (C₁-C₆)alkanoyl, α-amino(C₁-C₄)alkanyl, arylacyl and α-aminoacyl, or α-aminoacyl-α-aminoacyl, where each α-aminoacyl group is independently selected from the naturally occurring L-amino acids, P(O)(OH)₂, —P(O)(O(C₁-C₆)alkyl)₂ or glycosyl (the radical resulting from the removal of a hydroxyl group of the hemiacetal form of a carbohydrate), and the like.

If a compound used in the methods of the invention incorporates an amine functional group, a prodrug can be formed by the replacement of a hydrogen atom in the amine group with a group such as, for example, R-carbonyl, RO-carbonyl, NRR′-carbonyl where R and R′ are each independently (C₁-C₁₀)alkyl, (C₃-C₇) cycloalkyl, benzyl, or R-carbonyl is a natural α-aminoacyl or natural α-aminoacyl, —C(OH)C(O)OY¹ wherein Y¹ is H, (C₁-C₆)alkyl or benzyl, —C(OY²)Y³ wherein Y² is (C₁-C₄) alkyl and Y³ is (C₁-C₆)alkyl, carboxy (C₁-C₆)alkyl, amino(C₁-C₄)alkyl or mono-N— or di-N,N—(C₁-C₆)alkylaminoalkyl, —C(Y⁴)Y⁵ wherein Y⁴ is H or methyl and Y⁵ is mono-N— or di-N,N—(C₁-C₆)alkylamino morpholino, piperidin-1-yl or pyrrolidin-1-yl, and the like.

One or more compounds used in the methods of the invention may exist in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like, and it is intended that the invention embrace both solvated and unsolvated forms. “Solvate” means a physical association of a compound of the invention with one or more solvent molecules. This physical association involves varying degrees of ionic and covalent bonding, including hydrogen bonding. In certain instances, the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. “Solvate” encompasses both solution-phase and isolatable solvates. Non-limiting examples of suitable solvates include ethanolates, methanolates, and the like. “Hydrate” is a solvate wherein the solvent molecule is H₂O.

One or more compounds used in the methods of the invention may optionally be converted to a solvate. Preparation of solvates is generally known. Thus, for example M. Caira et al, J. Pharmaceutical Sci., 1993, 3, 601-611, describe the preparation of the solvates of the antifungal fluconazole in ethyl acetate as well as from water. Similar preparations of solvates, hemisolvate, hydrates and the like are described by E. C. van Tonder et al, AAPS PharmSciTech., 5(1), article 12 (2004); and A. L. Bingham et al, Chem. Commun., 603-604 (2001). A typical, non-limiting, process involves dissolving the inventive compound in desired amounts of the desired solvent (organic or water or mixtures thereof) at a higher than ambient temperature, and cooling the solution at a rate sufficient to form crystals which are then isolated by standard methods. Analytical techniques such as, for example I. R. spectroscopy, show the presence of the solvent (or water) in the crystals as a solvate (or hydrate).

“Effective amount” or “therapeutically effective amount” is meant to describe an amount of compound or a composition used in the methods of the present invention effective in inhibiting the above-noted diseases or enzyme activity and thus producing the desired therapeutic, ameliorative, inhibitory or preventative effect.

Another embodiment provides pharmaceutically acceptable salts of the compounds to be used in the methods of the invention. Thus, reference to a compound used in the methods of the invention herein is understood to include reference to salts thereof, unless otherwise indicated. The term “salt(s)”, as employed herein, denotes acidic salts formed with inorganic and/or organic acids, as well as basic salts formed with inorganic and/or organic bases. In addition, when a compound of the invention contains both a basic moiety, such as, but not limited to a pyridine or imidazole, and an acidic moiety, such as, but not limited to a carboxylic acid, zwitterions (“inner salts”) may be formed and are included within the term “salt(s)” as used herein. Pharmaceutically acceptable (i.e., non-toxic, physiologically acceptable) salts are preferred, although other salts are also useful. Salts of the compounds used in the methods of the invention may be formed, for example, by reacting a compound of the invention with an amount of acid or base, such as an equivalent amount, in a medium such as one in which the salt precipitates or in an aqueous medium followed by lyophilization.

Exemplary acid addition salts include acetates, ascorbates, benzoates, benzenesulfonates, bisulfates, borates, butyrates, citrates, camphorates, camphorsulfonates, fumarates, hydrochlorides, hydrobromides, hydroiodides, lactates, maleates, methanesulfonates, naphthalenesulfonates, nitrates, oxalates, phosphates, propionates, salicylates, succinates, sulfates, tartarates, thiocyanates, toluenesulfonates (also known as tosylates,) and the like. Additionally, acids which are generally considered suitable for the formation of pharmaceutically useful salts from basic pharmaceutical compounds are discussed, for example, by P. Stahl et al, Camille G. (eds.) Handbook of Pharmaceutical Salts. Properties, Selection and Use. (2002) Zurich: Wiley-VCH; S. Berge et al, Journal of Pharmaceutical Sciences (1977), 66(1), 1-19; P. Gould, International J. of Pharmaceutics (1986), 33, 201-217; Anderson et al, The Practice of Medicinal Chemistry (1996), Academic Press, New York; and in The Orange Book (Food & Drug Administration, Washington, D.C. on their website). These disclosures are incorporated herein by reference thereto.

Exemplary basic salts include ammonium salts, alkali metal salts such as sodium, lithium, and potassium salts, alkaline earth metal salts such as calcium and magnesium salts, salts with organic bases (for example, organic amines) such as dicyclohexylamines, t-butyl amines, and salts with amino acids such as arginine, lysine and the like. Basic nitrogen-containing groups may be quarternized with agents such as lower alkyl halides (e.g. methyl, ethyl, and butyl chlorides, bromides and iodides), dialkyl sulfates (e.g. dimethyl, diethyl, and dibutyl sulfates), long chain halides (e.g. decyl, lauryl, and stearyl chlorides, bromides and iodides), aralkyl halides (e.g. benzyl and phenethyl bromides), and others.

All such acid salts and base salts are intended to be pharmaceutically acceptable salts within the scope of the invention and all acid and base salts are considered equivalent to the free forms of the corresponding compounds for purposes of the invention.

Another embodiment provides pharmaceutically acceptable esters of the compounds used in the methods of the invention. Such esters include the following groups: (1) carboxylic acid esters obtained by esterification of the hydroxy groups, in which the non-carbonyl moiety of the carboxylic acid portion of the ester grouping is selected from straight or branched chain alkyl (for example, acetyl, n-propyl, t-butyl, or n-butyl), alkoxyalkyl (for example, methoxymethyl), aralkyl (for example, benzyl), aryloxyalkyl (for example, phenoxymethyl), aryl (for example, phenyl optionally substituted with, for example, halogen, C₁₋₄alkyl, or C₁₋₄alkoxy or amino); (2) sulfonate esters, such as alkyl- or aralkylsulfonyl (for example, methanesulfonyl); (3) amino acid esters (for example, L-valyl or L-isoleucyl); (4) phosphonate esters and (5) mono-, di- or triphosphate esters. The phosphate esters may be further esterified by, for example, a C₁₋₂₀ alcohol or reactive derivative thereof, or by a 2,3-di (C₆₋₂₄)acyl glycerol.

As mentioned herein, another embodiment provides tautomers of the compounds of the invention to be used in the methods herein, and salts, solvates, esters and prodrugs of said tautomers. It shall be understood that all tautomeric forms of such compounds are within the scope of the compounds used in the methods of the invention. For example, all keto-enol and imine-enamine forms of the compounds, when present, are included in the invention.

The compounds used in the methods of the invention may contain asymmetric or chiral centers, and, therefore, exist in different stereoisomeric forms. It is intended that all stereoisomeric forms of the compounds used in the methods of the invention as well as mixtures thereof, including racemic mixtures, form part of the present invention. In addition, the present invention embraces use of all geometric and positional isomers. For example, if a compound used in the methods of the invention incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the invention.

Another embodiment provides for diastereomeric mixtures and individual enantiomers of the compounds used in the methods of the invention. Diastereomeric mixtures can be separated into their individual diastereomers based on their physical chemical differences by methods well known to those skilled in the art, such as, for example, by chromatography and/or fractional crystallization. Enantiomers can be separated by converting the enantiomeric mixture into a diastereomeric mixture by reaction with an appropriate optically active compound (e.g., chiral auxiliary such as a chiral alcohol or Mosher's acid chloride), separating the diastereomers and converting (e.g., hydrolyzing) the individual diastereomers to the corresponding pure enantiomers. Also, some of the compounds used in the methods of the invention may be atropisomers (e.g., substituted biaryls) and are considered as part of this invention. Enantiomers can also be separated by use of chiral HPLC column.

All stereoisomers (for example, geometric isomers, optical isomers and the like) of the compounds used in the methods of the invention (including those of the salts, solvates, esters and prodrugs of the compounds as well as the salts, solvates and esters of the prodrugs), such as those which may exist due to asymmetric carbons on various substituents, including enantiomeric forms (which may exist even in the absence of asymmetric carbons), rotameric forms, atropisomers, and diastereomeric forms, are contemplated as embodiments within the scope of this invention, as are positional isomers (such as, for example, 4-pyridyl and 3-pyridyl). (For example, if a compound of the invention incorporates a double bond or a fused ring, both the cis- and trans-forms, as well as mixtures, are embraced within the scope of the invention. Also, for example, all keto-enol and imine-enamine forms of the compounds are included in the methods of the invention).

Individual stereoisomers of the compounds of the invention may, for example, be substantially free of other isomers, or may be admixed, for example, as racemates or with all other, or other selected, stereoisomers. The chiral centers of the present invention can have the S or R configuration as defined by the IUPAC 1974 Recommendations. The use of the terms “salt”, “solvate”, “ester”, “prodrug” and the like, is intended to equally apply to the salt, solvate, ester and prodrug of enantiomers, stereoisomers, rotamers, tautomers, positional isomers, racemates or prodrugs of the inventive compounds.

Another embodiment provides isotopically-labelled compounds to be used in the methods the invention. Such compounds are identical to those recited herein, but for the fact that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, fluorine and chlorine, such as ²H, ³H, ¹³C, ¹⁴C, ¹⁵N, ¹⁸O, ¹⁷O, ³¹P, ³²P, ³⁵S, ¹⁸F, and ³⁶Cl, respectively.

Certain isotopically-labelled compounds of the invention (e.g., those labeled with ³H and ¹⁴C) are useful in compound and/or substrate tissue distribution assays. Tritiated (i.e., ³H) and carbon-14 (i.e., ¹⁴C) isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavier isotopes such as deuterium (i.e., ²H) may afford certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and hence may be preferred in some circumstances. Isotopically labelled compounds of the invention can generally be prepared by following procedures analogous to those disclosed in the Schemes and/or in the Examples hereinbelow, by substituting an appropriate isotopically labelled reagent for a non-isotopically labelled reagent.

In the compounds used in the methods of the invention, the atoms may exhibit their natural isotopic abundances, or one or more of the atoms may be artificially enriched in a particular isotope having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number predominantly found in nature. The present invention is meant to include all suitable isotopic variations of the compounds of the invention. For example, different isotopic forms of hydrogen (H) include protium (¹H) and deuterium (²H). The presence of deuterium in the compounds of the invention is indicated by “D”. Protium is the predominant hydrogen isotope found in nature. Enriching for deuterium may afford certain therapeutic advantages, such as increasing in vivo half-life or reducing dosage requirements, or may provide a compound useful as a standard for characterization of biological samples. Isotopically-enriched compounds of the invention can be prepared without undue experimentation by conventional techniques well known to those skilled in the art or by processes analogous to those described in the schemes and examples herein using appropriate isotopically-enriched reagents and/or intermediates.

Polymorphic forms of the compounds used in the methods of the invention, and of the salts, solvates, esters and prodrugs of the compounds of the invention, are intended to be included in the present invention.

Methods of Treatment

The present invention is directed to methods of blocking P. falciparum growth in an animal, preferably a human, comprising administering to the animal an inhibitor with possible dual-targeting activity of Plasmepsin IX and Plasmepsin X or a pharmaceutically acceptable salt thereof. Plasmepsin IX and Plasmepsin X are responsible for critical processing of multiple other proteins required for the parasite to productively invade and egress from various cell types along the pathway of its lifecycle.

In certain embodiments, the present invention may provide a method for treating malaria in an animal, preferably a human, comprising administering to the animal an inhibitor with dual-targeting activity of Plasmepsin IX and Plasmepsin X or a pharmaceutically acceptable salt thereof. The present invention is directed to methods of treatment of Plasmodium infections comprising administering to a subject in need thereof a compound described herein, or a pharmaceutically acceptable salt thereof. More specifically, the methods of the invention comprise administration of a compound of Formula (I), or a pharmaceutically acceptable salt thereof. In certain embodiments, the compounds of Formula (I), or a pharmaceutically acceptable salt thereof, are administered in the form of a pharmaceutical composition, further comprising a pharmaceutically acceptable carrier or excipient.

The present invention provides a method for treating a Plasmodium infection, or for treating malaria, or for possibly inhibiting plasmepsin X which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, said compound having the structural Formula (I), (IA), (IB), (IC) or (ID) described in the Summary of the Invention. In some embodiments, the compounds of Formula (I), (IA), (IB), (IC) or (ID), or pharmaceutically acceptable salts thereof, are administered with a pharmaceutically acceptable carrier, as a pharmaceutical composition. Also provided herein are various embodiments of these methods, as described, infra.

The invention also relates to the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically acceptable salt thereof for the possible inhibition of plasmepsin X activity, for treating a Plasmodium infection, or for treating malaria. The invention further relates to the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the possible inhibition of plasmepsin X activity, for treating a Plasmodium infection, or for treating malaria. The compounds of Formula (I), (IA), (IB), (IC) or (ID) or pharmaceutically acceptable salts thereof described in any of the embodiments of the invention herein are useful for any of the uses above.

The present invention provides a method for treating a Plasmodium infection, or for treating malaria, or for the possible inhibition of plasmepsin IX which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, said compound having the structural Formula (I) described in the Summary of the Invention. In some embodiments, the compounds of Formula (I), or pharmaceutically acceptable salts thereof, are administered with a pharmaceutically acceptable carrier, as a pharmaceutical composition. Also provided herein are various embodiments of these methods, as described, infra.

The invention also relates to the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically acceptable salt thereof for the possible inhibition of plasmepsin IX activity, for treating a Plasmodium infection, or for treating malaria. The invention further relates to the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the possible inhibition of plasmepsin IX activity, for treating a Plasmodium infection, or for treating malaria. The compounds of Formula (I), (IA), (IB), (IC) or (ID) or pharmaceutically acceptable salts thereof described in any of the embodiments of the invention herein are useful for any of the uses above.

The present invention provides a method for treating a Plasmodium infection, or for treating malaria, or for the possible inhibition of plasmepsin X and plasmepsin IX which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound, or a pharmaceutically acceptable salt thereof, said compound having the structural Formula (I) described in the Summary of the Invention. In some embodiments, the compounds of Formula (I), or pharmaceutically acceptable salts thereof, are administered with a pharmaceutically acceptable carrier, as a pharmaceutical composition. Also provided herein are various embodiments of these methods, as described, infra.

The invention also relates to the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically acceptable salt thereof for the possible inhibition of plasmepsin X and plasmepsin IX activity, for treating a Plasmodium infection, or for treating malaria. The invention further relates to the use of a compound of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for the possible inhibition of plasmepsin X and plasmepsin IX activity, for treating a Plasmodium infection, or for treating malaria. The compounds of Formula (I), (IA), (IB), (IC) or (ID) or pharmaceutically acceptable salts thereof described in any of the embodiments of the invention herein are useful for any of the uses above.

The methods of the present invention are useful for treating malaria in that they inhibit the onset, growth, or progression of the condition, ameliorate the symptoms of the condition, cause regression of the condition, cure the condition, or otherwise improve the general well-being of a subject afflicted with, or at risk of, contracting the condition. Thus, in accordance with the presently disclosed subject matter, the terms “treat”, “treating”, and grammatical variations thereof, as well as the phrase “method of treating”, are meant to encompass any desired therapeutic intervention, including but not limited to a method for treating an existing infection in a subject of infection, such as in a subject that has been exposed to a parasite as disclosed herein.

Embodiments of the invention also include one or more of the compounds of Formula (I), (IA), (IB), (IC) or (ID) or a pharmaceutically acceptable salt thereof (i) for use in, (ii) for use as a medicament or composition for, or (iii) for use in the preparation of a medicament for: (a) therapy (e.g., of the human body); (b) medicine; (c) inhibition of parasite/Plasmodium growth, (d) treatment or prophylaxis of infection by Plasmodium species; (e) reduction of the progression, onset or severity of pathological symptoms associated with Plasmodium infection and/or reduction of the likelihood of severe Plasmodium infection or, (f) treatment, prophylaxis of, or delay in the onset, severity, or progression of Plasmodium-associated disease(s), including, but not limited to: malaria.

Accordingly, another embodiment provides methods for the treatment of malaria or for the treatment of Plasmodium infection, comprising administration of combinations comprising an amount of at least one compound of Formula (I), (A), (IB), (IC) or (ID), or a pharmaceutically acceptable salt, solvate, ester or prodrug thereof, and an effective amount of one or more additional agents described below. In certain embodiments, described herein are methods for the treatment of malaria or for the treatment of Plasmodium infection, comprising administration of combinations comprising an amount of at least one compound of Formula (I), (A), (IB), (IC) or (ID), or a pharmaceutically acceptable salt, solvate, ester or prodrug thereof, and an effective amount of one or more additional anit-malarial agents. In certain embodiments, described herein are methods for the treatment of malaria by inhibition of plasmepsin X, IX and at least one other mechanism, comprising administration of combinations comprising an amount of at least one compound of Formula (I), (A), (IB), (IC) or (ID), or a pharmaceutically acceptable salt, solvate, ester or prodrug thereof, and an effective amount of one or more additional anit-malarial agents, wherein the additional anti-malarial agents act through a different mechanism than inhibiting plasmepsin IX or plasmepsin X. The pharmacological properties of the compounds of Formula (I), (IA), (IB), (IC) or (ID), or a pharmaceutically acceptable salt thereof may be confirmed by several pharmacological assays. Certain assays are exemplified herein.

Dosage and Administration

Another embodiment provides suitable dosages and dosage forms of the compounds used in the methods of the invention. Suitable doses for administering compounds used in the methods of the invention to patients may readily be determined by those skilled in the art, e.g., by an attending physician, pharmacist, or other skilled worker, and may vary according to patient health, age, weight, frequency of administration, use with other active ingredients, and/or indication for which the compounds are administered. Doses may range from about 0.001 to 500 mg/kg of body weight/day of the compound of the invention. In one embodiment, the dosage is from about 0.01 to about 25 mg/kg of body weight/day of a compound of the invention, or a pharmaceutically acceptable salt or solvate of said compound. In another embodiment, the quantity of active compound in a unit dose of preparation may be varied or adjusted from about 1 mg to about 100 mg, in specific embodiments from about 1 mg to about 50 mg, in specific embodiments from about 1 mg to about 25 mg, according to the particular application. In another embodiment, a typical recommended daily dosage regimen for oral administration can range from about 1 mg/day to about 500 mg/day, in specific embodiments 1 mg/day to 200 mg/day, in two to four divided doses.

As discussed above, the amount and frequency of administration of the compounds of the invention and/or the pharmaceutically acceptable salts thereof will be regulated according to the judgment of the attending clinician considering such factors as age, condition and size of the patient as well as severity of the symptoms being treated.

Liquid form preparations include solutions, suspensions and emulsions. As an example, may be mentioned water or water-propylene glycol solutions for parenteral injection or addition of sweeteners and opacifiers for oral solutions, suspensions and emulsions. Liquid form preparations may also include solutions for intranasal administration.

Aerosol preparations suitable for inhalation may include solutions and solids in powder form, which may be in combination with a pharmaceutically acceptable carrier, such as an inert compressed gas, e.g. nitrogen.

Also included are solid form preparations that are intended to be converted, shortly before use, to liquid form preparations for either oral or parenteral administration. Such liquid forms include solutions, suspensions and emulsions.

Another embodiment provides for use of compositions comprising a compound of Formula (I), (IA), (IB), (IC) or (ID), or a pharmaceutically acceptable salt thereof formulated for transdermal delivery. The transdermal compositions can take the form of creams, lotions, aerosols and/or emulsions and can be included in a transdermal patch of the matrix or reservoir type as are conventional in the art for this purpose.

Another embodiment provides for use of compositions comprising a compound of Formula (I), (IA), (IB), (IC) or (ID), or a pharmaceutically acceptable salt thereof formulated for subcutaneous delivery. Another embodiment provides for use of compositions suitable for oral delivery. In some embodiments, it may be advantageous for the pharmaceutical preparation comprising one or more compounds of Formula (I), (IA), (IB), (IC) or (ID), or a pharmaceutically acceptable salt thereof to be prepared in a unit dosage form. In such forms, the preparation is subdivided into suitably sized unit doses containing appropriate quantities of the active component, e.g., an effective amount to achieve the desired purpose. Each of the foregoing alternatives is considered as included in the various embodiments of the invention.

When used in combination with one or more additional therapeutic agents (“combination therapy”), the compounds used in the methods of this invention, i.e. the compounds of Formula (I), (A), (IB), (IC) or (ID), may be administered together or sequentially. When administered sequentially, compounds of the invention may be administered before or after the one or more additional therapeutic agents, as determined by those skilled in the art or patient preference.

If formulated as a fixed dose, such combination products employ the compounds of Formula (I), (IA), (IB), (IC) or (ID), or a pharmaceutically acceptable salt thereof within the dosage range described herein and the other pharmaceutically active agent or treatment within its dosage range.

Combination Therapy

Another embodiment provides for methods of treatment using pharmaceutically acceptable compositions comprising a compound of the invention, either as the neat chemical or optionally further comprising additional ingredients. Such compositions are contemplated for preparation and use alone or in combination therapy. For preparing pharmaceutical compositions from the compounds of the invention, inert, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, dispersible granules, capsules, cachets and suppositories. The powders and tablets may be comprised of from about 5 to about 95 percent active ingredient. Suitable solid carriers are known in the art, e.g., magnesium carbonate, magnesium stearate, talc, sugar or lactose. Tablets, powders, cachets and capsules can be used as solid dosage forms suitable for oral administration. Examples of pharmaceutically acceptable carriers and methods of manufacture for various compositions may be found in A. Gennaro (ed.), Remington's Pharmaceutical Sciences, 18^(th) Edition, (1990), Mack Publishing Co., Easton, Pa.

Non-limiting examples of additional drugs and active agents useful in combination therapies for the treatment of malaria, include the following: Coartem® (Novartis International AG, Basel, Switzerland; artemether+lumefantrine), Eurartesim® (Sigma-Tau Pharmaceuticals, Inc., Rome, Italy; dihydroartemisinin-piperaquine), Pyramax® (Shin Poong Pharmaceutical Co., Ltd., Seoul, Korea; pyronaridine-artesunate), ASAQ Winthrop® (Sanofi SA (Gentilly, France)/DNDi (Geneva, Switzerland); artesunate+amodiaquine), ASMQ (Cipla Limited (Mumbai, India)/DNDi, artesunate+mefloquine), SPAQ-CO™ (Guilin Pharmaceutical Co., Ltd. (Shanghai), amodiaquine+sulfadoxine, pyrimethamine), Artesun® (Guilin Pharmaceutical, artesunate), artemether, artesunate, dihydroartemisinin, lumefantrine, amodiaquine, mefloquine, piperaquine, quinine, chloroquine, atovaquone and proguanil and sulfadoxine-pyrimethamine, Tafenoquine (Glaxosmithkline), OZ439/PQP (Sanofi), OZ439/FQ (Sanofi), KAE609 (Novartis), KAF156 (Novartis), DSM265 (NIH/Takeda), and MK-4815 (Merck & Co., Inc., Powles et al., Antimicrobial Agents and Chemotherapy 56(5): 2414-2419(2012)). Additional examples of drugs and active agents useful in combination therapies for the treatment of malaria, include the following: Ganaplacide, MMV048 and M5717. Compounds described herein exhibit asexual stage parasite killing and have reasonable half-lives, and therefore have the potential to form part of a single-exposure radical cure (SERC). Compounds described herein have demonstrated excellent transmission-blocking activity in a Standard Membrane Feeding Assay and therefore likely block transmission to mosquitos. They also display liver schizonticidal activity and therefore would also be a suitable chemo-prophylactic. Compounds described herein having a mechanism of action targeting both PM IX and X that is distinct from other antimalarial agents, will potentially display very little risk of cross-resistance to other antimalarials. Furthermore, the dual targeting action of the compounds described herein are likely to mean a substantial barrier to resistance acquisition in the field. Selection of such additional active ingredients will be according to the diseases or disorders present for which treatment is desired, as determined by the attending physician or other health care provider.

Thus, the invention also provides methods of using the compounds of Formula (I), (IA), (IB), (IC) or (ID), or a pharmaceutically acceptable salt thereof for the possible inhibition of plasmepsin X, plasmepsin IX or plasmepsin X and IX, to treat Plasmodium infection or treat malaria wherein the method further comprises administering to a subject in need thereof, one or more additional anti-malarial agents. In some embodiments, the one or more additional anti-malarial agents are selected from the group consisting of: artemether, lumefantrine, dihydroartemisinin, piperaquine, pyronaridine, artesunate, amodiaquine, mefloquine, sulfadoxine, pyrimethamine, lumefantrine, quinine, chloroquine, atovaquone, and proguanil.

EXAMPLES

ACN=acetonitrile=MeCN AcOEt=ethylacetate DCM=dichloromethane DIAD=Diisopropyl azodicarboxylate DIEA=N, N-Diisopropylethylamine, or Hunig's base

DMF=N,N-Dimethylformamide

DMP=dimethyl phthalate EDC=EDCI=1-ethyl-3-(3-dimethylaminopropyl)carbodiimide EtOAc=ethyl acetate EtOH=ethanol h=hours H₂=hydrogen

HPLC=High Performance Liquid Chromatography HOBt=Hydroxybenzotriazole

LCMS=Liquid chromatography-mass spectrometry LHMDS=LiHMDS=lithium bis(trimethylsilyl)amide LiAlH₄=lithium aluminum hydride min=minutes Me=methyl MeOH═CH₃OH=methanol N₂=nitrogen NaBH₄=sodium borohydride NaH=sodium hydride Na₂SO₄=sodium sulfate NH₄Cl=Ammonium chloride Pd(dppf)Cl₂=[1,1′-Bis(diphenylphosphino)ferrocene]palladium(II) dichloride

SFC=Supercritical Fluid Chromatography

SiO₂=Silicon dioxide TBAF=Tetra-n-butylammonium fluoride TFA=trifluoroacetic acid THF=tetrahydrofuran

TMS=Trimethylsilyl

CDCl₃=heavy chloroform CD₃OD=heavy methanol 1 Standard atmosphere [atm]=101325 pascal [Pa]=14.6959488 psi

The meanings of the abbreviations in the nuclear magnetic resonance spectra are shown below: s=singlet, d=doublet, dd=double doublet, dt=double triplet, ddd=double double doublet, sept=septet, t=triplet, m=multiplet, br=broad, brs=broad singlet, q=quartet, J=coupling constant and Hz=hertz.

Several methods for preparing the compounds of this disclosure are described in the following Schemes and Examples. Starting materials and intermediates were purchased commercially from common catalog sources or were made using known procedures, or as otherwise illustrated. Some frequently applied routes to the compounds of Formula I are described in in the Schemes that follow. In some cases, the order of carrying out the reaction steps in the schemes may be varied to facilitate the reaction or to avoid unwanted reaction products. An asterisk (*) may be used in a chemical structure drawing that indicates the location of a chiral center.

Intermediate compounds of Formula S-2 are prepared from chiral epoxides S-1, in which P is an alcohol protecting group, after cyclopropanation conditions such as (EtO)₂POCH₂CO₂R/NaH followed by alcohol deprotection. Alcohol oxidation in S-2 can be performed using oxidants such as Dess-Martin peiodinane to yield aldehydes S-3. Treatment of S-3 with an organometallic reagent S-4, in which M is a metal and X is a halogen, such as organozinc (M=Zn) and organomagnesium (M=Mg) species gives secondary alcohol intermediates S-5. Iminopyrimidones or iminohydantoins S-6 are introduced under Mitsunobu conditions to give intermediates S-7. Alternatively, alcohol in intermediates S-5 could be transformed into a leaving group such as a mesylate, tosylate, triflate or halogen which can be displaced with intermeidates S-6 to give intermediates S-7. At this step diastereomeric mixtures can be separated by SFC and the resulting chiral esters subjected to acid or base catalyzed hydrolysis or hydrogenation to give chiral acid intermediates S-8. Finally, S-8 intermediates are coupled with amines S-7 to provide final products of Formula S-10 after iminopyrimidone deprotection using acids as TFA or Zn.

Reactions sensitive to moisture or air were performed inside a glove-box or under nitrogen or argon using anhydrous solvents and reagents. The progress of reactions was determined by either analytical thin layer chromatography (TLC) usually performed with E. Merck pre-coated TLC plates, silica gel 60F-254, layer thickness 0.25 mm or liquid chromatography-mass spectrometry (LC/MS).

Typically, the analytical LC-MS system used consisted of a Waters ZQ™ platform with electrospray ionization in positive ion detection mode with an Agilent 1100 series HPLC with autosampler. The column was commonly a Waters Xterra MS C18, 3.0×50 mm, 5 μm or a Waters Acquity UPLC© BEH C18 1.0×50 mm, 1.7 μm. The flow rate was 1 mL/min, and the injection volume was 10 μL. UV detection was in the range 210-400 nm. The mobile phase consisted of solvent A (water plus 0.05% TFA) and solvent B (MeCN plus 0.05% TFA) with a gradient of 100% solvent A for 0.7 min changing to 100% solvent B over 3.75 min, maintained for 1.1 min, then reverting to 100% solvent A over 0.2 min.

Preparative HPLC purifications were usually performed using either a mass spectrometry directed system or a non-mass guided system. Usually they were performed on a Waters Chromatography Workstation configured with LC-MS System consisting of: Waters ZQ™ single quad MS system with Electrospray Ionization, Waters 2525 Gradient Pump, Waters 2767 Injecto/Collector, Waters 996 PDA Detector, the MS Conditions of: 150-750 amu, Positive Electrospray, Collection Triggered by MS, and a Waters SUNFIRE© C-18 5-micron, 30 mm (id)×100 mm column. The mobile phases consisted of mixtures of acetonitrile (10-100%) in water containing 0.1% TFA. Flow rates were maintained at 50 mL/min, the injection volume was 1800 μL, and the UV detection range was 210-400 nm. An alternate preparative HPLC system used was a Gilson Workstation consisting of: Gilson GX-281 Injector/Collector, Gilson UV/VIS-155 Detector, Gilson 333 and 334 Pumps, and either a Phenomenex Gemini-NX C-18 5-micron, 50 mm (id)×250 mm column or a Waters XBridge™ C-18 5-micron OBD™, 30 mm (id)×250 mm column. The mobile phases consisted of mixtures of acetonitrile (0-75%) in water containing 5 mmol (NH₄)HCO₃. Flow rates were maintained at 50 mL/min for the Waters Xbridge™ column and 90 mL/min for the Phenomenex Gemini column. The injection volume ranged from 1000-8000 μL, and the UV detection range was 210-400 nm. Mobile phase gradients were optimized for the individual compounds. Reactions performed using microwave irradiation were normally carried out using an Emrys Optimizer manufactured by Personal Chemistry, or an Initiator manufactured by Biotage. Concentration of solutions was carried out on a rotary evaporator under reduced pressure. Flash chromatography was usually performed using either a Biotage© Flash Chromatography apparatus (Dyax Corp.), an ISCO CombiFlash® Rf apparatus, or an ISCO CombiFlash® Companion XL on silica gel (32-63 μM, 60 Å pore size) in pre-packed cartridges of the size noted. ¹H NMR spectra were acquired at 500 MHz spectrometers in CDCl₃ solutions unless otherwise noted. Chemical shifts were reported in parts per million (ppm). Tetramethylsilane (TMS) was used as internal reference in CDCl₃ solutions, and residual CH₃OH peak or TMS was used as internal reference in CD₃OD solutions. Coupling constants (J) were reported in hertz (Hz). Chiral analytical chromatography was most commonly performed on one of CHIRALPAK© AS, CHIRALPAK©AD, CHIRALCEL© OD, CHIRALCEL©IA, or CHIRALCEL© OJ columns (250×4.6 mm) (Daicel Chemical Industries, Ltd.) with noted percentage of either ethanol in hexane (% Et/Hex) or isopropanol in heptane (% IPA/Hep) as isocratic solvent systems. Chiral preparative chromatography was conducted on one of CHIRALPAK AS, of CHIRALPAK AD, CHIRALCEL© OD, CHIRALCEL©IA, CHIRALCEL© OJ columns (20×250 mm) (Daicel Chemical Industries, Ltd.) with desired isocratic solvent systems identified on chiral analytical chromatography or by supercritical fluid (SFC) conditions. It is understood that a chiral center in a compound may exist in the “S” or “R”

stereo-configuration, or as a mixture of both. Within a molecule, each bond drawn as a straight line from a chiral center includes both the (R) and (S) stereoisomers as well as mixtures thereof.

Intermediate 1

(1R,2R)-ethyl 2-(hydroxymethyl)cyclopropanecarboxylate Step 1

Ethyl 2-diethoxyphosphorylacetate (546 g, 2.44 mol, 2.0 eq) was added dropwise to a suspension of NaH (97.4 g, 2.44 mol, 60% purity, 2.0 eq) in toluene (2.0 L) at 5° C. The resulting mixture was stirred for 30 min at 5° C. Then (S)-2-((benzyloxy)methyl)oxirane (compound 1) (200 g, 1.22 mol, 1.0 eq) was added to the mixture at 5° C. and the resulting mixture was stirred for 12 h at 95° C. Then, the mixture was poured into sat.NH₄Cl (30 L) at 0° C. under N₂, and the aqueous phase was extracted with EtOAc (4 L×2). The combined organic layer was washed with brine (3.0 L) and dried with Na₂SO₄, filtered, and the solvent evaporated in vacuo to give a residue that was purified by silica gel chromatography (SiO₂, petroleum ether:EtOAc=10:0 to 0:1) to give compound 2.

H-NMR: (400 MHz, DMSO): δ: 7.26-7.37 (m, 5H), 4.47 (s, 2H), 4.02-4.08 (m, 2H), 3.44-3.48 (m, 1H), 3.28-3.31 (m, 1H), 1.55-1.59 (m, 2H), 1.16-1.20 (m, 3H), 1.03-1.04 (m, 1H), 0.88-0.89 (m, 1H).

Step 2

A solution of compound 2 (100 g, 362.80 mmol, 1 eq) in EtOH (1000 mL) was added into a hydrogenation bottle previously charged with Pd/C (20 g, 10% purity) at 25° C. The mixture was purged with H₂ three times and stirred under H₂ (30 Psi) at 25° C. for 24 h. Then the mixture was filtered and the volatiles removed in vacuo to give a residue that was purified by silica gel chromatography (SiO₂, Petroleum ether:EtOAc=10:1 to 0:1) to give Intermediate 1.

H-NMR: (400 MHz, CDCl₃): δ: 4.10-4.17 (m, 2H), 3.61-3.64 (m, 1H), 3.48-3.51 (m, 1H), 1.57-1.78 (m, 3H), 1.21-1.28 (m, 4H), 0.86-0.88 (m, 1H).

Example 1

Step 1

TBAF (155 mL, 155 mmol) was added to a solution of (1R,2R)-ethyl 2-(((tert-butyldimethylsilyl)oxy)methyl)cyclopropanecarboxylate (20 g, 77 mmol) in THF (150 mL) and the mixture was stirred at 26° C. for 2 hours. The mixture was diluted with water (300 mL), extracted with EtOAc (400 mL×3). The organic layers were washed with brine (500 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by column chromatography (Petroleum ether/EtOAc=100:1-10:1) to afford product (1R,2R)-ethyl 2-(hydroxymethyl)cyclopropanecarboxylate.

¹H NMR (400 MHz, chloroform-d) δ=4.22-4.05 (m, 2H), 3.64 (td, J=5.9, 11.6 Hz, 1H), 3.55-3.42 (m, 1H), 1.80-1.68 (m, 1H), 1.57 (td, J=4.4, 8.5 Hz, 1H), 1.49 (t, J=5.7 Hz, 1H), 1.32-1.18 (m, 4H), 0.87 (ddd, J=4.4, 6.2, 8.4 Hz, 1H)

Step 2

DMP (46.3 g, 109 mmol) was added to a solution of (1R,2R)-ethyl 2-(hydroxymethyl)cyclopropanecarboxylate (10.5 g, 72.8 mmol) in DCM (400 mL). The mixture was stirred at 25° C. for 10 h under N₂ atmosphere. The mixture was filtered, then concentrated and purified by flash column (Petroleum ether/EtOAc=100:0 to 10:1) to afford product (1R,2R)-ethyl 2-formylcyclopropanecarboxylate.

¹H NMR (500 MHz, chloroform-d) δ=9.31 (s, 1H), 4.15-4.22 (m, 2H), 2.39-2.49 (m, 1H), 2.06-2.26 (m, 1H), 1.60-1.62 (m, 1H), 1.50-1.51 (m, 1H), 1.27-1.30 (m, 3H)

Step 3

Zinc (4.60 g, 70.3 mmol) was added to a solution of (1R,2R)-ethyl 2-formylcyclopropanecarboxylate (5 g, 35.2 mmol) and 3-iodoprop-1-ene (8.86 g, 52.8 mmol) in THE (80 mL) under N₂. The mixture was stirred at 25° C. for 12 h. The mixture was filtered, and the filtrate was concentrated. The residue was purified by column chromatography (Petroleum ether/EtOAc=30:1-5:1) to afford product (1R,2R)-ethyl 2-(1-hydroxybut-3-en-1-yl)cyclopropanecarboxylate.

¹H NMR (500 MHz, chloroform-d) δ=5.76-5.94 (m, 1H), 5.08-5.23 (m, 2H), 4.07-4.23 (m, 2H), 3.15-3.40 (m, 1H), 2.26-2.49 (m, 2H), 1.58-1.67 (m, 2H), 1.26-1.28 (mz, 3H), 1.25-1.26 (m, 1H), 0.84-1.03 (m, 1H)

Step 4

Tertbutylchlorodiphenylsilane (5.97 g, 21.71 mmol) and imidazole (2.217 g, 32.6 mmol) were added to a solution of (1R,2R)-ethyl 2-(1-hydroxybut-3-en-1-yl)cyclopropanecarboxylate (2 g, 10.86 mmol) in DCM (70 mL), and the mixture was stirred at 25° C. for 12 hours under N₂ atmosphere. The mixture was diluted with water (50 mL), extracted with DCM (50 mL×3). The organic layers were washed with brine (50 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by column chromatography (Petroleum ether/EtOAc=100:1-50:1) to afford product (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)but-3-en-1-yl)cyclopropanecarboxylate.

¹H NMR (400 MHz, chloroform-d) δ 7.62-7.79 (m, 4H), 7.31-7.50 (m, 6H), 5.69-5.94 (m, 1H), 4.90-5.07 (m, 2H), 3.86-4.15 (m, 2H), 3.16-3.38 (m, 1H), 2.31 (s, 2H), 1.57-1.69 (m, 1H), 1.32-1.42 (m, 1H), 1.14-1.29 (m, 4H), 1.03-1.05 (m, 10H), 0.81-0.99 (m, 1H), 0.47-0.71 (m, 1H)

Step 5

2,6-dimethylpyridine (2.370 mL, 20.35 mmol), osmium tetroxide (0.160 mL, 0.509 mmol) and sodium periodate (8.70 g, 40.7 mmol) were added to a solution of (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)but-3-en-1-yl)cyclopropanecarboxylate (4.3 g, 10.17 mmol) in 1,4-dioxane (90 mL) and water (30 mL). The mixture was stirred at 25° C. for 2 h. The mixture was quenched with water (80 mL), then extracted with EtOAc (100 mL×3). The organic layers were washed with brine (100 mL), dried over Na₂SO₄, filtered and concentrated in vacuo to give (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)-3-oxopropyl)cyclopropanecarboxylate, which was used for the next step directly.

¹H NMR (500 MHz, chloroform-d) δ 9.75-9.83 (m, 1H), 7.68-7.74 (m, 3H), 7.65 (dd, J=1.5, 8.0 Hz, 1H), 7.41-7.48 (m, 3H), 7.38-7.41 (m, 3H), 4.06-4.10 (m, 1H), 3.80-4.01 (m, 1H), 3.55-3.77 (m, 1H), 2.56-2.77 (m, 2H), 1.63-1.77 (m, 1H), 1.17-1.42 (m, 4H), 1.02 (d, J=16.0 Hz, 10H), 0.46-0.64 (m, 1H)

Step 6

NaBH₄ (0.766 g, 20.25 mmol) was added to solution of (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)-3-oxopropyl)cyclopropanecarboxylate (4.3 g, 0.00 mmol) in MeOH (50 mL). The mixture was stirred at −40° C. for 10 min. The mixture was then diluted with water (60 mL) and extracted with EtOAc (80 mL×3). The organic layers were washed with brine (80 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by column chromatography (Petroleum ether/EtOAc=20:1) to afford product (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)-3-hydroxypropyl)cyclopropanecarboxylate.

H NMR (500 MHz, chloroform-d) δ 7.67-7.75 (m, 4H), 7.37-7.48 (m, 6H), 4.07 (q, J=7.0 Hz, 1H), 3.85-3.99 (m, 1H), 3.69-3.85 (m, 2H), 3.25-3.42 (m, 1H), 1.78-1.94 (m, 2H), 1.61-1.74 (m, 2H), 1.29-1.32 (m, 0.5H), 1.14-1.24 (m, 3H), 1.08-1.11 (m, 0.5H), 1.04 (d, J=15.0 Hz, 10H), 0.35-0.58 (m, 1H)

Step 7

Trimethyloxonium tetrafluoroborate (1.352 g, 9.14 mmol) and 1,8-bis(dimethylamino)naphthalene (2.61 g, 12.19 mmol) was added to a solution of (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)-3-hydroxypropyl)cyclopropanecarboxylate (1.3 g, 3.05 mmol) in DCM (50 mL). The mixture was stirred at 25° C. for 13 hrs. The mixture was diluted with water (30 mL) and extracted with DCM (30 mL×3). The organic layers were washed with brine (40 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by Prep.TLC (Petroleum ether/EtOAc=20:1) to afford product (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)-3-methoxypropyl)cyclopropanecarboxylate.

¹H NMR (500 MHz, chloroform-d) δ 7.62-7.82 (m, 4H), 7.31-7.51 (m, 6H), 4.04-4.13 (m, 1H), 3.76-4.00 (m, 1H), 3.42-3.57 (m, 2H), 3.20-3.34 (m, 4H), 1.73-2.01 (m, 2H), 1.60-1.67 (m, 0.5H), 1.31-1.34 (m, 0.5H), 1.12-1.25 (m, 3H), 0.99-1.03 (m, 10H), 0.79-0.91 (m, 1H), 0.58-0.59 (m, 0.5H), 0.36-0.40 (m, 0.5H)

Step 8

TBAF (6.99 mL, 6.99 mmol) was added to a solution of (1R,2R)-ethyl 2-(1-((tert-butyldiphenylsilyl)oxy)-3-methoxypropyl)cyclopropanecarboxylate (1.54 g, 3.49 mmol) in THE (20 mL). The mixture was stirred at 26° C. for 2 hours. The mixture was then diluted with water (20 mL) and extracted with EtOAc (20 mL×3). The organic layers were washed with brine (30 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by column chromatography (Petroleum ether/EtOAc=20:1-2:1) to afford product (1R,2R)-ethyl 2-(1-hydroxy-3-methoxypropyl)cyclopropanecarboxylate.

¹H NMR (500 MHz, chloroform-d) δ 4.08-4.18 (m, 2H), 3.67 (td, J=5.0, 9.5 Hz, 1H), 3.53-3.62 (m, 1H), 3.40-3.53 (m, 1H), 3.36 (d, J=2.0 Hz, 3H), 2.90 (d, J=2.0 Hz, 1H), 1.81-1.91 (m, 2H), 1.58-1.73 (m, 2H), 1.27 (dt, J=2.5, 7.0 Hz, 3H), 1.17 (qd, J=4.5, 16.5 Hz, 1H), 0.86-1.05 (m, 1H)

Step 9

(E)-diisopropyl diazene-1,2-dicarboxylate (0.916 mL, 4.65 mmol) was added dropwise, at 0° C., under N₂ atmosphere to a solution of (E)-tert-butyl (4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (626 mg, 2.324 mmol), (1R,2R)-ethyl 2-(1-hydroxy-3-methoxypropyl)cyclopropanecarboxylate (470 mg, 2.324 mmol) and triphenylphosphine (1219 mg, 4.65 mmol) in THF (15 mL). The mixture was stirred at 25° C. for 12 h. The mixture was then concentrated in vacuo. The residue was purified by column chromatography (Petroleum ether:EtOAc=15:1-5:1) to afford product (1R,2R)-ethyl 2-(1-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)cyclopropanecarboxylate. MS (ESI) m z 454.3 (M+H⁺)

Step 10

(1R,2R)-ethyl 2-(1-(2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)cyclopropanecarboxylate (800 mg, 1.429 mmol) was separated by SFC (Instrument SFC-16 Method Column Phenomenex-Cellulose-2 (250 mm*30 mm, 5 um) Condition 0.1% NH₃H₂O EtOH Begin B 15% End B 15% Gradient Time (min) 100% B Hold Time (min) FlowRate (mL/min) 60 Injections 150) to afford product (1R,2R)-ethyl 2-((R)-1-(2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)cyclopropanecarboxylate (Peak 1, Rt=2.072) and (1R,2R)-ethyl 2-((R)-1-(2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)cyclopropanecarboxylate (Peak 2, Rt=2.356) both as a colorless oil.

MS (ESI) m z 454.3 (M+H⁺).

Peak 1: ¹H NMR (500 MHz, chloroform-d) δ=9.60-10.17 (m, 1H), 4.36-4.52 (m, 1H), 3.97-4.14 (m, 2H), 3.32-3.53 (m, 2H), 3.28 (s, 3H), 2.50-2.63 (m, 3H), 2.30-2.45 (m, 1H), 2.01-2.19 (m, 1H), 1.60-1.66 (m, 5H), 1.39-1.52 (m, 9H), 1.19-1.21 (m, 4H), 0.87-1.03 (m, 7H)

Peak 2: ¹H NMR (500 MHz, chloroform-d) δ=9.72-10.07 (m, 1H), 4.45 (s, 1H), 4.12-4.13 (m, 2H), 3.39-3.42 (m, 2H), 3.33 (s, 3H), 2.56 (s, 2H), 2.23-2.42 (m, 2H), 1.62 (s, 5H), 1.46-1.52 (m, 9H), 1.24-1.27 (m, 4H), 0.86-1.04 (m, 7H)

Step 11

Lithium hydroxide monohydrate (486 mg, 11.57 mmol) was added to a solution of (1R,2R)-ethyl 2-((R)-1-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)cyclopropanecarboxylate (350 mg, 0.772 mmol) in MeOH (6 mL), THE (6 mL) and water (1.2 mL). The mixture was stirred at 30° C. for 4 hours. EtOAc (4 mL) was added to the mixture and the mixture was neutralized to pH 5-6 with citric acid. The mixture was then quenched with water (10 mL), and extracted with EtOAc (15 mL×3). The organic layers were washed with brine (15 mL), dried over Na₂SO₄, filtered, and concentrated in vacuo to afford crude product (1R,2R)-2-((R)-1-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)cyclopropanecarboxylic acid, which was used for next step directly.

MS (ESI) m/z 426.2 (M+H⁺).

Step 12

DIEA (0.205 mL, 1.175 mmol) was added to a solution of (1R,2R)-2-((R)-1-(2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)cyclopropanecarboxylic acid (100 mg, 0.00 mmol), (S)-2,2-dimethylchroman-4-amine hydrochloride (60.3 mg, 0.282 mmol), HOBt (72.0 mg, 0.470 mmol) and EDCI (180 mg, 0.940 mmol) in THE (6 mL). The mixture was stirred at 25° C. for 2 hours. The mixture was quenched with water (10 mL), and extracted with EtOAc (10 mL×3). The organic layers were washed with brine (15 mL), dried over Na₂SO₄, filtered, and concentrated in vacuo to afford crude product, then purified by Prep-TLC (Petroleum ether/EtOAc=1:2) to give the product tert-butyl (1-((R)-1-((1R,2R)-2-(((S)-2,2-dimethylchroman-4-yl)carbamoyl)cyclopropyl)-3-methoxypropyl)-4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate.

MS (ESI) m z 585.4 (M+H⁺).

Step 13

TFA (4 mL) at 22° C. was added to a solution of tert-butyl (1-((R)-1-((1R,2R)-2-(((S)-2,2-dimethylchroman-4-yl)carbamoyl)cyclopropyl)-3-methoxypropyl)-4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (100 mg, 0.171 mmol) in DCM (12 mL). The reaction was stirred for 1 h. The mixture was concentrated in vacuo, purified by HPLC (Boston Green ODS 150*30 5u, Condition water (0.1% TFA)-ACN, Begin B 33, End B 63, Gradient Time (min) 10, 100% B Hold Time (min) 2, FlowRate (mL/min) 25, Injections 3), then lyphilized to give the product (1R,2R)-2-((R)-1-(4,4-diethyl-2-imino-6-oxotetrahydropyrimidin-1(2H)-yl)-3-methoxypropyl)-N—((S)-2,2-dimethylchroman-4-yl)cyclopropanecarboxamide. MS (ESI) m z 485.4 (M+H⁺).

Example 1: ¹H NMR (500 MHz, METHANOL-d4) δ=8.50-8.23 (m, 1H), 7.15-7.14 (m, 1H), 7.08 (d, J=7.5 Hz, 1H), 6.88 (dt, J=1.0, 7.5 Hz, 1H), 6.74 (dd, J=1.0, 8.0 Hz, 1H), 5.37-5.18 (m, 1H), 3.58-3.44 (m, 2H), 3.39 (s, 1H), 3.35 (s, 3H), 2.89 (d, J=16.0 Hz, 1H), 2.70 (d, J=16.0 Hz, 1H), 2.51 (s, 2H), 2.58-2.40 (m, 1H), 2.22 (m, 1H), 2.14-2.02 (m, 1H), 1.88-1.50 (m, 6H), 1.44 (s, 3H), 1.35 (m, 1H), 1.32-1.25 (m, 3H), 0.99-0.71 (m, 6H).

Example 2

Example 2 Step 1

TBAF (232 mL, 232 mmol) was added to a solution of (1R,2R)-ethyl 2-(((tert-butyldimethylsilyl)oxy)methyl)cyclopropanecarboxylate (30 g, 116 mmol) in THE (150 mL). The mixture was stirred at 26° C. for 2 hours. The mixture was diluted with water (300 mL), and extracted with EtOAc (400 mL×3). The organic layers were washed with brine (500 mL), dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by column chromatography (Petroleum ether/EtOAc=100:1-10:1) to afford product (1R,2R)-ethyl 2-(hydroxymethyl)cyclopropanecarboxylate.

¹H NMR (500 MHz, chloroform-d) δ=4.19-4.07 (m, 2H), 3.63 (td, J₁=5.0, J₂=11.0 Hz, 1H), 3.54-3.43 (m, 1H), 1.72-1.73 (m, 1H), 1.56-1.58 (m, 2H), 1.31-1.25 (m, 3H), 1.22-1.23 (m, 1H), 0.92-0.82 (m, 1H).

Step 2

DMP (70.6 g, 166 mmol) was added to a solution of (1R,2R)-ethyl 2-(hydroxymethyl)cyclopropanecarboxylate (16 g, 111 mmol) in DCM (600 mL). The mixture was stirred under nitrogen at 20° C. for 10 h. The mixture was filtered, then concentrated and purified by flash column (Petroleum ether/EtOAc=100:0 to 10:1) to afford product (1R,2R)-ethyl 2-formylcyclopropanecarboxylate.

¹H NMR (500 MHz, chloroform-d) δ=9.31 (d, J=4.0 Hz, 1H), 4.27-4.15 (m, 2H), 2.41-2.44 (m, 1H), 2.32-2.22 (m, 1H), 1.61-1.62 (m, 1H), 1.57-1.49 (m, 1H), 1.30-1.27 (m, 3H)

Step 3

Isopropylmagnesium lithium chloride (30.3 mL, 39.4 mmol) was added dropwise to a solution of 3-iodopyridine (8.08 g, 39.4 mmol) and zinc(II) chloride (1.970 mL, 1.970 mmol) in THE (50 mL) at 0° C. under N₂ atmosphere. Then the mixture was stirred at 15° C. for 20 min. Then the mixture was cooled to −20° C. (1R,2R)-ethyl 2-formylcyclopropanecarboxylate (4 g, 19.70 mmol) in THE (20 mL) was added to the mixture dropwise. The mixture was stirred at −20° C. for 1 h. The mixture was quenched with sat. a.q. NH₄Cl (40 mL), and extracted with EtOAc (50 mL×3). The combined organic layers were washed with brine (50 mL), dried over Na₂SO₄, filtered, concentrated in vacuo. The residue was purified by flash column (Petroleum ether/EtOAc=10:1 to 1:1) to afford (1R,2R)-ethyl 2-(hydroxy(pyridin-3-yl)methyl)cyclopropanecarboxylate. MS (ESI) m z 222.1 (M+H⁺).

¹H NMR (500 MHz, chloroform-d) δ 8.57 (s, 1H), 8.51 (td, J₁=1.5 Hz, J₂=5.0 Hz, 1H), 7.84-7.72 (m, 1H), 7.31 (td, J₁=4.0 Hz, J₂=8.0 Hz, 1H), 4.52 (d, J=6.0 Hz, 1H), 4.29 (d, J=7.0 Hz, 1H), 4.16-4.03 (m, 2H), 1.89-1.76 (m, 2H), 1.29-1.22 (m, 4H), 1.19-1.11 (m, 1H), 1.06-0.96 (m, 1H)

Step 4

(E)-diisopropyl diazene-1,2-dicarboxylate (1.247 mL, 6.33 mmol) was added dropwise to a solution of (E)-tert-butyl (4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (0.852 g, 3.16 mmol), (1R,2R)-ethyl 2-(hydroxy(pyridin-3-yl)methyl)cyclopropanecarboxylate (0.7 g, 3.16 mmol) and triphenylphosphine (1.660 g, 6.33 mmol) in THE (20 mL) at 0° C. under N₂ atmosphere. Then the mixture was stirred at 23° C. for 12 h. Water (20 mL) was added to the mixture, and extracted with EtOAc (15 mL×3). The combined organic layers were washed with brine (15 mL), dried over Na₂SO₄, filtered, concentrated in vacuo. The residue was purified by column chromatography (Petroleum ether:EtOAc=10:1-5:1), then purified by Prep-TLC (Petroleum ether:EtOAc=3:2) to afford (1R,2R)-ethyl 2-(((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)cyclopropanecarboxylate. MS (ESI) m z 473.3 (M+H⁺).

¹H NMR (500 MHz, METHANOL-d₄) δ=8.55 (d, J=2.0 Hz, 1H), 8.45-8.37 (m, 1H), 7.83 (d, J=8.5 Hz, 1H), 7.46-7.34 (m, 1H), 5.66-5.51 (m, 1H), 4.23-4.12 (m, 2H), 2.69 (s, 2H), 1.78-1.58 (m, 5H), 1.55-1.41 (m, 10H), 1.32-1.16 (m, 7H), 1.13-1.04 (m, 1H), 1.01-0.85 (m, 7H)

Step 5

(1R,2R)-ethyl 2-((S)-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)cyclopropanecarboxylate (440 mg, 0.931 mmol) was separated by SFC (Instrument SFC 13 Method Column DAICEL CHIRALPAK IC (250 mm*30 mm, 5 um) Condition 0.1% NH₃H₂O EtOH Begin B 35% End B 35% Gradient Time (min) 100% B Hold Time (min) FlowRate (mL/min) 60 Injections 120) to afford product (1R,2R)-ethyl 2-((S)-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)cyclopropanecarboxylate (Peak 1, Rt=3.403) and (1R,2R)-ethyl 2-((S)-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)cyclopropanecarboxylate (Peak 2, Rt=4.649).

MS (ESI) m z 473.3 (M+H⁺)

Step 6

Lithium hydroxide monohydrate (124 mg, 2.96 mmol) was added to a solution of (1R,2R)-ethyl 2-((S)-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)cyclopropanecarboxylate (140 mg, 0.296 mmol) in MeOH (4 mL), THE (4 mL) and water (0.4 mL). The mixture was stirred at 25° C. for 2 hours. EtOAc (4 mL) was added to the mixture and the mixture was neutralized to pH 5-6 with citric acid. The mixture was quenched with water (10 mL), and extracted with EtOAc (15 mL×3). The organic layers were washed with brine (15 mL), dried over Na₂SO₄, filtered, and concentrated in vacuo to afford crude product (1R,2R)-2-((S)-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)cyclopropanecarboxylic acid, which was used for next step directly. MS (ESI) m z 445.2 (M+H⁺)

Step 7

DIEA (0.196 mL, 1.125 mmol) was added to a solution of (1R,2R)-2-((S)-((E)-2-((tert-butoxycarbonyl)imino)-4,4-diethyl-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)cyclopropanecarboxylic acid (100 mg, crude), (S)-2,2-dimethylchroman-4-amine (61.7 mg, 0.225 mmol), HOBt (68.9 mg, 0.450 mmol), EDCI (86 mg, 0.450 mmol) in THF (8 mL). The mixture was stirred at 22° C. for 12 hours. The mixture was quenched with water (15 mL), and extracted with EtOAc (15 mL×3). The organic layers were washed with brine (15 mL), dried over Na₂SO₄, filtered, and concentrated in vacuo to afford crude product, then purified by Prep-TLC (Petroleum ether/EtOAc=1:2) to give the product tert-butyl (1-((S)-((1R,2R)-2-(((S)-2,2-dimethylchroman-4-yl)carbamoyl)cyclopropyl)(pyridin-3-yl)methyl)-4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate. MS (ESI) m z 604.3 (M+H⁺)

Step 8

TFA (2 mL) was added to a solution of tert-butyl (1-((S)-((1R,2R)-2-(((S)-2,2-dimethylchroman-4-yl)carbamoyl)cyclopropyl)(pyridin-3-yl)methyl)-4,4-diethyl-6-oxotetrahydropyrimidin-2(1H)-ylidene)carbamate (90 mg, 0.149 mmol) in DCM (6 mL). The reaction was stirred at 23° C. for 1 h. The mixture was concentrated in vacuo, then purified by HPLC (Instrument ee Method Column YMC-Actus Pro C18 150*30 5u Condition water (0.1% TFA)-ACN Begin B 24 End B 54 Gradient Time (min) 11 100% B Hold Time (min) 1.1 FlowRate (mL/min) 40 Injections 3) to give the product (1R,2R)-2-((S)-(4,4-diethyl-2-imino-6-oxotetrahydropyrimidin-1(2H)-yl)(pyridin-3-yl)methyl)-N—((S)-2,2-dimethylchroman-4-yl)cyclopropanecarboxamide. MS (ESI) m z 504.2 (M+H⁺).

¹H NMR (500 MHz, METHANOL-d₄) δ 8.85 (s, 1H), 8.66 (s, 1H), 8.58 (d, J=8.5 Hz, 1H), 8.29 (d, J=8.0 Hz, 1H), 7.74 (s, 1H), 7.17-7.08 (m, 2H), 6.89-6.82 (m, 1H), 6.74 (d, J=8.0 Hz, 1H), 5.25-5.18 (m, 1H), 4.95 (d, J=3.0 Hz, 1H), 2.98-2.90 (m, 1H), 2.87-2.66 (m, 2H), 2.09-2.11 (in, 1H), 1.85-1.92 (m, 1H), 1.82 (t, J=12.5 Hz, 1H), 1.75-1.66 (m, 3H), 1.65-1.56 (m, 2H), 1.43 (s, 3H), 1.29 (s, 3H), 1.21 (s, 1H), 0.92 (t, J=7.5 Hz, 3H), 0.83 (t, J=7.5 Hz, 3H)

The compounds in Table 1 and 2 were made in accordance with the general schemes and Example 1 and 2 described above.

TABLE 1 LC/MS Ex. Structure (M + 1)+  1A

485.3  1B

485.4  2

504.3  3A

371.1  3B

371.3  4A

399.2  4B

399.2  4C

399.3  4D

399.2  5

427.3  6A

399.1  6B

399.2  7A

445.3  7B

445.3  8A

467.3  8B

467.3  9

476.3  10

476.3  11A

520.3  11B

520.3  12

544.3  13

457.4  14

525.3  15A

435.2  15B

435.2  16A

463.3  16B

463.3  17A

503.2  17B

503.2  18A

479.2  18B

479.3  19A

485.3  19B

485.4  20

485.3  21A

525.2  21B

525.2  22

457.2  23A

457.3  23B

457.3  24A

497.2  24B

497.2  24C

497.1  25A

441.3  25B

441.3  26

476.3  27

492.3  28A

481.3  28B

481.3  29A

427.3  29B

427.3  29C

427.3  30A

457.3  30B

457.3  31A

495.3  31B

495.2  32

516.2  33

413.3  34

455.3  35A

443.3  35B

443.1  36A

427.3  36B

427.3  37

546.2  38

506.3  39

522.3  40

506.2  41

546.1  42

522.2  43A

544.2  43B

544.2  44A

572.2  44B

572.3  45A

500.2  45B

500.2  46

516.2  47A

540.2  47B

540.1  48A

532.3  48B

532.3  49A

504.3  49B

504.2  50

500.2  51

516.2  52

540.2  53A

518.2  53B

518.3  54A

530.2  54B

530  55A

558.3  55B

558  56A

518.1  56B

518.1  57A

490.3  57B

490.1  58A

510.2  58B

510.2  59

526.2  60A

550.2  60B

550.2  61

487.3  62A

511.3  62B

511.3  63

490.3  64A

471.3  64B

471.3  65A

506.3  65B

506.3  66

524.3  67A

508.3  67B

508.3  68

548.2  69A

526.2  69B

526.3  69C

526.3  69D

526.2  69E

526.2  70A

487.3  70B

487.3  71

417.2  72A

475.3  72B

475.3  73

429.3  74A

487.3  74B

487.3  75A

471.3  75B

471.3  75C

471.3  75D

471.3  76A

475.3  76B

475.3  77A

494.3  77B

494.3  78

413.3  79A

427.3  79B

427.3  79C

427.3  80A

485.4  80B

485.3  80C

485.3  80D

485.3  81A

504.4  81B

504.3  81C

504.4  81D

504.4  82A

487.3  82B

487.3  82C

487.3  82D

487.3  83A

471.3  83B

471.3  84

413.2  85A

490.3  85B

490.3  86A

494.3  86B

494.3  87

417.3  88A

475.3  88B

475.3  89A

491.3  89B

491.3  90

433.2  91A

510.3  91B

510.3  92A

485.3  92B

485.4  93A

494.3  93B

494.3  94A

544.2, 546.2  94B

544.2, 546.2  95

433.2  96A

506.3  96B

506.3  97A

467.1, 469.2  97B

467.1, 469.2  98A

494.3  98B

494.3  99

485.2 100

467.2 101

491.3 102A

508.2 102B

508.2 103A

538.2 103B

538.3 104A

564.2 104B

564.2 105A

490.3 105B

490.3 105C

490.3 105D

490.3 106A

525.2, 527.2 106B

525.2, 527.2 107A

541.3 107B

541.3 108A

506.3 108B

506.2 108C

506.3 108D

506.3 109A

544.3 109B

544.3 110A

525.3 110B

525.3 111A

475.3 111B

475.3 112A

491.3 112B

491.3 113A

493.3 113B

493.3 114A

543.3 114B

543.2 115A

562.2 115B

562.2 116A

491.2 116B

491.3 117A

510.2 117B

510.3 118

483.2 119

433.2 120

510.3 121A

510.2 121B

510.3 122A

512.2 122B

512.2 123

485.3 124

505.3 125A

483.1 125B

483.1 126A

541.3 126B

541.3 127A

539.3 127B

539.3 128

413.3 129A

555.3 129B

555.2 130A

489.3 130B

489.2 131A

469.2 131B

469.3 132

471.3 133A

399.3 133B

399.2 134A

541.1 134B

541.1 135

411.2 136

447.3 137A

505.3 137B

505.3 138A

485.2 138B

485.2 139A

403.2 139B

403.1 140A

411.1 140B

411.2

TABLE 2 LC/MS Ex. Structure (M + 1)+ 141

485.3 142A

499.4 142B

499.4 142C

499.4 142D

499.3 143A

499.4 143B

499.4 143C

499.4 144

501.3 145

501.1 146

519.3 147A

519.1 147B

519.1 148A

519.1 148B

519.1 149A

519.3 149B

519.3 150

535.2

Assessing Antiparasite Potency in a Parasite LDH Growth Assay (Parasite Assay)

The parasite stock was maintained at 400 haematocrit in RPMI-Hepes media buffered with sodium bicarbonate and supplemented with 500 heat inactivated human serum and 0.500 albumax.

Approximately 42 hours prior to the potency assay being set up, parasites were synchronized with 500 sorbitol to select for ring stage parasites. On the day of assay set up, a blood smear of the parasite culture was Giemsa stained and counted. The parasitemia was adjusted to 0.7% rings and the haematocrit was diluted to 2% in RPMI-Hepes media buffered with sodium bicarbonate and supplemented with 500 heat inactivated human serum and 0.5% albumax. 30 ul of diluted parasites are then added into 10 ul of media+compound in pre-prepared Greiner TC assay plates. Parasite assay plates were placed in gassed humidified boxes in single layer and allowed to incubate at 37° C. for 72 hours. After 72 hours growth, assay plates are sealed with parafilm and frozen flat, in single file at −80° C. overnight. On the following day, assay plates are allowed to thaw at room temperature for 4 hours to which an LDH assay is performed to measure parasite growth.

Assay EC₅₀ results are shown in Table 3 and Table 4.

TABLE 3 Example EC₅₀ (nM)  1A 1  1B 250  2 0.9  3A 234  3B 151  4A 94  4B 26  4C 85  4D 91  5 49  6A 188  6B 269  7A 135  7B 29  8A 37  8B 5.3  9 6.9  10 27  11A 4.1  11B 24  12 0.9  13 24  14 0.9  15A 240  15B 230  16A 33  16B 119  17A 47  17B 20  18A 39  18B 180  19A 28  19B 4.2  20 5.3  21A 260  21B 1  22 44  23A 16  23B 15  24A 3.4  24B 2  24C 120  25A 84  25B 25  26 4.7  27 78  28A 12  28B 14  29A 203  29B 351  29C 113  30A 45  30B 7.7  31A 215  31B 213  32 1.8  33 76  34 286  35A 85  35B 220  36A 247  36B 301  37 1.2  38 6.5  39 10.1  40 3.1  41 1.1  42 18.4  43A 4  43B 37  44A 8.3  44B 0.9  45A 2.8  45B 220  46 2.5  47A 155  47B 0.9  48A 2.7  48B 7.4  49A 20  49B 14  50 3  51 5.3  52 1  53A 3.1  53B 2.1  54A 1  54B 130  55A 0.8  55B 180  56A 65  56B 0.7  57A 3.2  57B 100  58A 210  58B 1  59 3.8  60A 0.5  60B 100  61 6.9  62A 31  62B 0.8  63 1.2  64A 2.3  64B 195  65A 2.8  65B 8  66 2.6  67A 0.9  67B 0.6  68 0.3  69A 1  69B 1.9  69C 0.8  69D 0.3  69E 300  70A 77  70B 19  71 125  72A 11  72B 2.5  73 192  74A 33  74B 3.1  75A 8.2  75B 7.5  75C 7  75D 7  76A 17  76B 15  77A 1.7  77B 2.3  78 300  79A 255  79B 275  79C 289  80A 9.4  80B 6.6  80C 14  80D 21  81A 2.7  81B 1  81C 0.9  81D 1.3  82A 24.4  82B 57  82C 53  82D 27  83A 6.6  83B 26  84 289  85A 2.4  85B 0.9  86A 2.2  86B 2.8  87 160  88A 5.2  88B 10  89A 5.6  89B 22  90 225  91A 0.8  91B 2.7  92A 7.6  92B 7.2  93A 3.3  93B 2.4  94A 0.9  94B 0.9  95 110  96A 2.7  96B 3.3  97A 180  97B 220  98A 0.9  98B 2.2  99 130 100 170 101 2.9 102A 0.8 102B 2.5 103A 29 103B 2.1 104A 4 104B 22 105A 0.9 105B 1.8 105C 0.8 105D 0.9 106A 7.4 106B 10 107A 2.1 107B 11 108A 70 108B 3.7 108C 6 108D 8.5 109A 2.3 109B 1 110A 21 110B 7.2 111A 24 111B 15 112A 18 112B 9.4 113A 19 113B 7.5 114A 89 114B 5.1 115A 6.6 115B 1 116A 0.8 116B 18 117A 0.8 117B 2.7 118 63 119 26 120 1 121A 2.5 121B 2 122A 2.3 122B 2.2 123 2 124 0.8 125A 231 125B 160 126A 4.7 126B 8.4 127A 1.1 127B 2.6 128 280 129A 1.3 129B 2.5 130A 0.9 130B 2.4 131A 6.8 131B 6.1 132 6.7 133A 210 133B 100 134A 71 134B 25 135 190 136 160 137A 12 137B 6.4 138A 7.4 138B 18 139A 99 139B 130 140A 220 140B 280

TABLE 4 141 4.7 142A 1.9 142B 0.7 142C 204.9 142D 114.9 143A 4.6 143B 2.4 143C 57.9 144 164.9 145 8.4 146 1.1 147A 2.8 147B 8.2 148A 2.7 148B 1.9 149A 1.0 149B 1.2 150 3.9 

1. A compound having the structural Formula (I):

or a pharmaceutically acceptable salt thereof, wherein: R¹ is a heterocycloalkyl, C₃-C₁₂cycloalkyl, aryl or C₁-C₆alkylaryl, wherein the heterocycloalkyl, C₃-C₁₂cycloalkyl, aryl, C₁-C₆alkylaryl is unsubstituted or substituted with 1 to 5 substituents independently selected from the group consisting of halogen, CN, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, oxo, COOC₁-C₆alkyl, C₃-C₆cycloalkyl, spiroC₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) and C₁-C₆alkylN(R⁷)(R⁸); R² is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH; R³ is hydrogen, halogen, CN, OH, C₃-C₆cycloalkyl or C₁-C₆alkyl; R⁴ is hydrogen, halogen, CN, OH, C₃-C₆cycloalkyl or C₁-C₆alkyl; R⁵ is hydrogen, halogen, CN, OH, alkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) or C₁-C₆alkylN(R⁷)(R⁸) or when taken with R⁶ forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl; R⁶ is hydrogen, halogen, CN, OH, alkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) or C₁-C₆alkylN(R⁷)(R⁸) or when taken with R⁵ forms a C₃-C₆cycloalkyl or C₃-C₆heterocycloalkyl; R⁷ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH; R⁸ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH; and R⁹ is hydrogen, halogen, CN, alkoxy, C₁-C₆alkyl, heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl, aryl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸), wherein the C₁-C₆alkyl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl, aryl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸), and wherein the heterocycloalkyl, heteroaryl, C₃-C₁₂cycloalkyl and aryl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy, C₁-C₆alkyl, COOH, oxo, COOC₁-C₆alkyl, haloC₁-C₆alkyl, OH, CON(R⁷)(R⁸) and N(R⁷)(R⁸).
 2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R¹ is selected from the group consisting of:

wherein R¹ is unsubstituted or substituted with 1 to 5 substituents selected from the group consisting of halogen, CN, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, C₁-C₆alkylCOOH, COOH, oxo, COOC₁-C₆alkyl, C₃-C₆cycloalkyl, spiroC₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl, C₁-C₆alkylOH, CON(R⁷)(R⁸), N(R⁷)(R⁸) and C₁-C₆alkylN(R⁷)(R⁸); R⁷ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH; and R⁸ is hydrogen, C₁-C₆alkylCOOH, COOH, C₃-C₆cycloalkyl, C₁-C₆alkyl, haloC₁-C₆alkyl or C₁-C₆alkylOH.
 3. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R¹ is:

wherein R¹ is unsubstituted or substituted with 1 to 5 substituents selected from the group consisting of halogen, CN, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, oxo, COOC₁-C₆alkyl, C₁-C₆alkyl, haloC₁-C₆alkyl and C₁-C₆alkylOH.
 4. The compound of claims 1-2, or a pharmaceutically acceptable salt thereof, wherein R¹ is:

wherein R¹ is unsubstituted or substituted with 1 to 5 substituents selected from the group consisting of halogen, CN, OH, alkoxy, haloalkoxy, C₁-C₆alkylOC₁-C₆alkyl, oxo, COOC₁-C₆alkyl, C₁-C₆alkyl, haloC₁-C₆alkyl C₃-C₆cycloalkyl, spiroC₃-C₆cycloalkyl, and C₁-C₆alkylOH.
 5. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R² is hydrogen.
 6. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R¹ is a chromane or indane moiety.
 7. The compound of claim 6, or a pharmaceutically acceptable salt thereof, wherein R¹ is:


8. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R³ is selected from hydrogen, halogen or C₁-C₆alkyl.
 9. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R⁴ is selected from hydrogen, halogen or C₁-C₆alkyl.
 10. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R⁵ and R⁶ are independently selected from hydrogen or C₁-C₆alkyl.
 11. (canceled)
 12. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R⁵ and R⁶ are independently selected from methyl or ethyl.
 13. (canceled)
 14. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R⁹ is hydrogen, C₁-C₆alkyl, heteroaryl, or aryl, wherein the C₁-C₆alkyl, heteroaryl or aryl can be unsubstituted or substituted with one, two or three substituents selected from the group consisting of halogen, CN, OH, alkoxy.
 15. A compound selected from the group consisting of:

or a pharmaceutically acceptable salt thereof.
 16. A pharmaceutical composition comprising a compound of claim 1 or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
 17. (canceled)
 18. A method for treating a Plasmodium infection, or for treating malaria, which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound of claim 1, or a pharmaceutically acceptable salt thereof.
 19. A method for inhibiting plasmepsin X which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound of claim 1, or a pharmaceutically acceptable salt thereof.
 20. A method for inhibiting plasmepsin IX which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound of claim 1, or a pharmaceutically acceptable salt thereof.
 21. A method for dual inhibition of plasmepsin IX and plasmepsin X which comprises administering to a subject in need of such treatment a therapeutically effective amount of a compound of claim 1, or a pharmaceutically acceptable salt thereof. 22-25. (canceled)
 26. A method for treating a Plasmodium infection, or for treating malaria, comprising administration of a compound of claim 1, or a pharmaceutically acceptable salt thereof, and an effective amount of one or more additional anti-malarial agents.
 27. A method for the treatment of malaria by inhibition of plasmepsin X, IX and at least one other mechanism, comprising administration of a compound of claim 1, or a pharmaceutically acceptable salt thereof, and an effective amount of one additional anti-malarial agent, wherein the additional anti-malarial agent acts through a different mechanism than inhibiting plasmepsin IX or plasmepsin X. 